Difference between revisions of "Team:TCU Taiwan/Result/Peptides"

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To indirect prove wound healing, we perform experiments in cell migration. Two cells are selected for the experiment: Squamous keratinocyte called HaCat, and Dermal microvascular endothelium cell called HMEC-1. To make the result closer to human, both cells were sampled from human cells.</br>
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Squamous keratinocyt cell shows the influence of our peptides on epithelial. On other hand, because angiogenesis is one of the indicators of wound healing, Dermal microvascular endothelium cell is selected here.</br>
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In cell migration experiment, samples are divided into following groups:</br>1. Mock</br>2. pQE-60 has no insert and no IPTG induction of LB medium for 200 <span style="font-family:Symbol;"> </span>l  (Control 1)</br>3. pQE-60 has insert, without IPTG induction of LB medium for 200 <span style="font-family:Symbol;"> </span>l (Control 2)</br>4. pQE-60 has no insert, with IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l (Control 3)</br>5. 1<span style="font-family:Symbol;"> </span>l AMPs </br>Furthermore, all of above groups are divided into three more groups: Epinecidin-1, Signiferin, Epinecidin-1 and Signiferin.</br>6. 5<span style="font-family:Symbol;"> </span>l AMPs</br>7. 100<span style="font-family:Symbol;"> </span>l AMPs</br>8. 200<span style="font-family:Symbol;"> </span>l AMPs</br>
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Revision as of 22:29, 18 September 2015

To indirect prove wound healing, we perform experiments in cell migration. Two cells are selected for the experiment: Squamous keratinocyte called HaCat, and Dermal microvascular endothelium cell called HMEC-1. To make the result closer to human, both cells were sampled from human cells.
Squamous keratinocyt cell shows the influence of our peptides on epithelial. On other hand, because angiogenesis is one of the indicators of wound healing, Dermal microvascular endothelium cell is selected here.
In cell migration experiment, samples are divided into following groups:
1. Mock
2. pQE-60 has no insert and no IPTG induction of LB medium for 200  l (Control 1)
3. pQE-60 has insert, without IPTG induction of LB medium for 200  l (Control 2)
4. pQE-60 has no insert, with IPTG induction of LB medium for 200 l (Control 3)
5. 1 l AMPs
Furthermore, all of above groups are divided into three more groups: Epinecidin-1, Signiferin, Epinecidin-1 and Signiferin.
6. 5 l AMPs
7. 100 l AMPs
8. 200 l AMPs


             
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