Difference between revisions of "Team:BostonU/Mammalian synbio/Current Challenges"
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− | <p style="padding-bottom:60px;">Here, we have presented some key problems or issues that we believe might currently be surrounding mammalian synthetic biology work in the iGEM community. Read on further to look at some of the preliminary solutions | + | <p style="padding-bottom:60px;">Here, we have presented some key problems or issues that we believe might currently be surrounding mammalian synthetic biology work in the iGEM community. Read on further to look at some of the preliminary <a href="https://2015.igem.org/Team:BostonU/Mammalian_synbio/Solutions"style="color:#FF9966;">solutions we have proposed</a> as a first step in thinking about this challenge.</p> |
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Latest revision as of 22:29, 18 September 2015
Significance | Current Challenges | Proposed Solutions |
Current Challenges
We aimed to identify a few broad reasons why mammalian cells are not heavily used in the iGEM community.
Cost
We very quickly recognized that there is a high cost associated with performing research in mammalian cells. This cost comes at many levels.
- Higher Cost of Equipment and Facilities
To use classic mammalian cell lines such as HEK293T cells, researchers require BSL2 certified spaces. The space itself may be difficult for individuals to obtain - working with multiple organisms in the same lab space may result in more chances for contamination, and thus for best results, researchers may want to have a designated space for mammalian use. Our iGEM team stems from a lab that has many researchers working with mammalian cells, and we were fortunate to be able to use dedicated BSL2 facilities for our research project.
Some notable facilities and equipment needed for mammalian cell work include:
- CO2 Incubator: In order to keep mammalian cells alive and growing, they need to be incubated at a certain temperature and CO2 level. Our incubator was set at 37 C with a CO2 level of 5.0%.
- O2 Tissue Culture Hood: It is imperative to work in a sterile environment when working with mammalian cells to prevent contamination. The tissue culture hood provided this sterile environment for us to transfect, passage, and plate mammalian cells in. The tissue culture hood provides laminar flow of air to keep objects sterile, and also includes UV light sterilization after usage.
- Tissue Culture Plates: these are sterile plates upon which we plate mammalian cells for growth and transfection. The plates are treated with polystyrene such that cells can adhere on the bottom and spread out.
- Higher Cost of Upkeep of Cells
Mammalian cell lines require a lot of upkeep. To do transient transfection, cells need to be in an actively dividing state. However, if cells are left in the same tissue culture dish for a few days, they will revert into a quiescent state and will not be adequate for experimental purposes. Thus, researchers need to passage cells every 3-4 days, diluting them and transferring them into new dishes or flasks so that they are actively dividing and healthy.
The process of cell passaging requires expensive reagents such as:
- FBS (fetal bovine serum) - promotes growth of mammalian cell culture
- DMEM (Dulbecco’s Modified Eagle Modicum) - media that supports growth of mammalian cell culture
- Penicillin Streptomycin (Pen-Strep) - helps prevent contamination in mammalian cell culture
- L-glutamine - amino acid that promotes cell growth in mammalian cell culture
- Sodium pyruvate - acts as an additional source of carbon and glucose for cell
- Trypsin - protease that cleaves mammalian cell surface peptides to release adherent cells from a tissue culture treated plate
- DPBS (Dulbecco’s Phosphate-Buffered Saline) - provides a buffer system to maintain media at optimal conditions
We purchased most of these Corning products from Fisher Scientific.
Parts
Because sharing and submission of standardized parts to the common Registry is a core mission of iGEM, we sought to identify how mammalian part submissions might play a role in hindering teams from using mammalian cells.
- Lower Number of Registry Parts
We searched through the iGEM registry for functional parts that we could potentially use in our mammalian systems. iGEM teams generally tend to work in E. coli because it is easy to work with, grows quickly, and functions as a good host for foreign DNA sequences. Because of this, the vast majority of parts are designed to operate in E. coli. There is not a significant number of parts designed for optimal function in mammalian chassis. As a result, it is difficult for teams that are working with mammalian chassis to use and characterize old parts, as many of may not have full functionality in mammalian systems. This dissuades many teams from using mammalian chassis.
- More Difficult Submission Standards
There are two current avenues for teams performing mammalian research to submit their parts to the iGEM registry.
The first is to submit parts as classic BioBricks. This ensures that parts are cloned and submitted in the pSB1C3 backbone for consistent iGEM handling, as well as that parts are flanked by proper restriction sites for subsequent cloning. One issue with this standard is that mammalian parts are quite large, and with many internal restriction sites it is difficult to remove these so that they are easily cloned as BioBricks.
Our team noticed this problem during our own project. In order to clone some of our parts (~1-5kB) as BioBricks, we found many instances of 2-3 internal EcoRI, SpeI, XbaI, and PstI sites in these parts. We are thankful for the IDT offer that enabled us to eliminate some of these sites and synthesize our parts such that they were BioBrick compatible.
The other option for part submission is to use the MammoBlock standard proposed by the MIT 2010 and 2011 teams. MammoBlocks utilizes a recombination-based assembly and is able to bypass the original BioBrick conformity requirements. However, cloning MammoBlock parts requires Gateway assembly, which is expensive and proprietary, and may therefore be infeasible for many teams.
- Lower Knowledge of Research
For our team, we were fortunate to receive a lot of guidance and resources from our lab members. In particular, our PIs Dr. Mo Khalil and Dr. Wilson Wong are very interested in studying cutting-edge eukaryotic synthetic biology systems, particularly for translational health and therapeutic applications.
When a new team begins their iGEM journey, they may be inspired by past projects. Many of these notable examples stem from engineering classic microbes, such as E. coli, and these sources of inspiration serve as an important platform for how teams develop new project ideas.
We think that if the iGEM community had the opportunity to learn more about emerging mammalian synthetic biology research and applications, this may inspire more future iGEM teams to attempt this research.
- Lower Incentive or Reward
We more broadly thought of this problem in terms of how certain chassis may be well-suited or more optimal for different projects and applications, and furthermore if iGEM teams are hindered from pursuing these particular projects from purely a cost or resource standpoint. For example, teams that are interested in health and medicine applications and how their projects perform in a mammalian cell may not gravitate towards actually using this chassis because there is no incentive or reward for doing so, and the high cost vs. low resource dilemma may be too high of a barrier. Such a team might instead settle on a “less optimal” chassis. In contrast, teams that do actually perform research in the most optimal possible chassis may have a slight advantage with judging, but are not necessarily formally rewarded for doing so.
Awareness
We recognized that overall awareness about mammalian synthetic biology efforts may be limited in the iGEM community.
Here, we have presented some key problems or issues that we believe might currently be surrounding mammalian synthetic biology work in the iGEM community. Read on further to look at some of the preliminary solutions we have proposed as a first step in thinking about this challenge.