Difference between revisions of "Team:WLC-Milwaukee/Parts"
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<p>Of all the tolC proteins we used in our project, three were ultimately submitted to the BioBrick registry. These submitted parts were tolC homologs from Yersenia pestis Angola (BBa_K1683000), Proteus mirabilis (BBa_K1683002), and Pseudomonas aeruginosa PA01 (oprM) (BBa_K168004). These proteins were submitted as a part of larger constructs which we used to express usable tolC molecules in our chassis organism, ΔtolC E. coli. These constructs consist of 4 major components. First is the pBAD promoter, an arabinose-induced strong E. coli promoter. Second is the strong E. coli ribosomal binding site (originally from BBa_B0034). Third is the E. coli tolC signaling sequence; this forms a chimeric protein with the tolC or tolC homolog of interest. See Project > Solution > TolC for more information on TolC.</p> | <p>Of all the tolC proteins we used in our project, three were ultimately submitted to the BioBrick registry. These submitted parts were tolC homologs from Yersenia pestis Angola (BBa_K1683000), Proteus mirabilis (BBa_K1683002), and Pseudomonas aeruginosa PA01 (oprM) (BBa_K168004). These proteins were submitted as a part of larger constructs which we used to express usable tolC molecules in our chassis organism, ΔtolC E. coli. These constructs consist of 4 major components. First is the pBAD promoter, an arabinose-induced strong E. coli promoter. Second is the strong E. coli ribosomal binding site (originally from BBa_B0034). Third is the E. coli tolC signaling sequence; this forms a chimeric protein with the tolC or tolC homolog of interest. See Project > Solution > TolC for more information on TolC.</p> | ||
<p>These parts were characterized by western blotting and through Kirby-Bauer antibiotic assays. More detailed analysis is found on the Documentation page’s Results section. The important snippets relating to the submitted parts have been extracted for this page.</p> | <p>These parts were characterized by western blotting and through Kirby-Bauer antibiotic assays. More detailed analysis is found on the Documentation page’s Results section. The important snippets relating to the submitted parts have been extracted for this page.</p> | ||
| − | <table style="width: | + | <table style="width:80%"> <tr><td width="33%"><img src="https://static.igem.org/mediawiki/2015/c/c2/WLC-YP-WESTERN.jpeg"></td> |
<td width="33%"><img src="https://static.igem.org/mediawiki/2015/3/32/WLC-PROT-WESETRN.jpeg"></td> | <td width="33%"><img src="https://static.igem.org/mediawiki/2015/3/32/WLC-PROT-WESETRN.jpeg"></td> | ||
<td width="33%"><img src="https://static.igem.org/mediawiki/2015/b/b4/WLC-PSD-WESTERN.jpeg"></td> | <td width="33%"><img src="https://static.igem.org/mediawiki/2015/b/b4/WLC-PSD-WESTERN.jpeg"></td> | ||
| − | </tr><tr><td width="33%"><img src="https://static.igem.org/mediawiki/2015/5/54/WLC-YP-KB.jpeg"></td> | + | </tr><tr><td width="33%"><img width="500px" src="https://static.igem.org/mediawiki/2015/5/54/WLC-YP-KB.jpeg"></td> |
| − | <td width="33%"><img src="https://static.igem.org/mediawiki/2015/3/3a/WLC-PROT-KB.jpeg"></td> | + | <td width="33%"><img width="500px" src="https://static.igem.org/mediawiki/2015/3/3a/WLC-PROT-KB.jpeg"></td> |
| − | <td width="33%"><img src="https://static.igem.org/mediawiki/2015/4/48/WLC-PSD-KB.jpeg"></td> | + | <td width="33%"><img width="500px" src="https://static.igem.org/mediawiki/2015/4/48/WLC-PSD-KB.jpeg"></td> |
</tr></table> | </tr></table> | ||
</div> | </div> | ||
Revision as of 22:30, 18 September 2015
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Parts
Of all the tolC proteins we used in our project, three were ultimately submitted to the BioBrick registry. These submitted parts were tolC homologs from Yersenia pestis Angola (BBa_K1683000), Proteus mirabilis (BBa_K1683002), and Pseudomonas aeruginosa PA01 (oprM) (BBa_K168004). These proteins were submitted as a part of larger constructs which we used to express usable tolC molecules in our chassis organism, ΔtolC E. coli. These constructs consist of 4 major components. First is the pBAD promoter, an arabinose-induced strong E. coli promoter. Second is the strong E. coli ribosomal binding site (originally from BBa_B0034). Third is the E. coli tolC signaling sequence; this forms a chimeric protein with the tolC or tolC homolog of interest. See Project > Solution > TolC for more information on TolC.
These parts were characterized by western blotting and through Kirby-Bauer antibiotic assays. More detailed analysis is found on the Documentation page’s Results section. The important snippets relating to the submitted parts have been extracted for this page.
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