MEETING 1 23/06/15

Attendees

Suparna, Natalie, Moyra, Richard, Robert, James, Alice, Anna, Katarina, Ben, Morena and Mark

Overall plan

Use the pVS72 plasmid from VS45 strain (ΔCsgAB) and insert it into the pSBIC3 plasmid in MS348 strain (by Gibson assembly or conventional cloning) to be submitted to iGEM. The pVS72 contains an N-terminal CsgA signal sequence attached to Sup35NM (codes the amyloid protein), which is attached to the gene coding for Cytb₅₆₂. The sup35NM monomers should assemble above the export system on the cell surface. The cytochrome binds haem cofactor, once outside the cell, which will help to conduct electrons. Ultimate goal would be to have the electrons coming from the electron transport chain (ETC) into the biofilm and going to an electron acceptor.

Modelling

Validation

Application of project

Entrepreneurship competition

MEETING 2 30/06/15

Set up Top10 overnight, VS45 overnight, US348 overnight

Meeting with advisors to discuss progress

MEETING 3 7/07/15

MEETING 4 14/07/15

MEETING 5 21/07/15

MEETING 6 28/07/15

MEETING 7 04/08/15

MEETING 8 11/08/15

MEETING 9 18/08/15

MEETING 10 25/08/15

MEETING 11 01/09/15

MEETING 12 08/09/15

MEETING 13 15/09/15

MEETING 14 22/09/15