Difference between revisions of "Team:RHIT/Protocol"

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   <li class="steps">Spin and resuspend cells in 0.5 ml 1 x TE.</li>
 
   <li class="steps">Spin and resuspend cells in 0.5 ml 1 x TE.</li>
 
   <li class="steps">Place 100 - 200 µl per plate and incubate at 30℃ for three days.</li>
 
   <li class="steps">Place 100 - 200 µl per plate and incubate at 30℃ for three days.</li>
 +
<p>http://research.fhcrc.org/breeden/en/methods.html</p>
 
   </ol>
 
   </ol>
 
</ul>
 
</ul>

Revision as of 22:37, 18 September 2015

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Protocols

  • QIAprep Spin Miniprep Kit

  • NEBuilder HiFi DNA Assembly Reaction Protocol

  • Boiling Prep Protocol

  • NEB High Efficiency Transformation Protocol

  • QIAquick Gel Extraction Kit

  • Ethanol Precipitation

  • Yeast Transformation (From Breeden Lab)