Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/10 May 2015"

(Created page with "='''Transformation with pET24a'''= 1. Thaw 50uL of chemically competent cells for 10 minutes on ice. 2. Add 1uL of pET expression vector to cells, incubate for 5 minutes. 3. Res...")
 
(→‎Transformation with pET24a)
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='''Transformation with pET24a'''=
 
='''Transformation with pET24a'''=
  
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1. Thaw 50uL of chemically competent cells for 10 minutes on ice.
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#Thaw 50uL of chemically competent cells for 10 minutes on ice.
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2. Add 1uL of pET expression vector to cells, incubate for 5 minutes.
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#Add 1uL of pET expression vector to cells, incubate for 5 minutes.
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3. Rescue in 200uL of SOC, incubate at 37C for 1 hour.
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# Rescue in 200uL of SOC, incubate at 37C for 1 hour.
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4. Plate 100uL on kanamycin plates, spread with beads.
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# Plate 100uL on kanamycin plates, spread with beads.
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5. Incubate at 37C.
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# Incubate at 37C.

Revision as of 05:03, 8 July 2015

Transformation with pET24a

  1. Thaw 50uL of chemically competent cells for 10 minutes on ice.
  2. Add 1uL of pET expression vector to cells, incubate for 5 minutes.
  3. Rescue in 200uL of SOC, incubate at 37C for 1 hour.
  4. Plate 100uL on kanamycin plates, spread with beads.
  5. Incubate at 37C.