Difference between revisions of "Team:Uppsala/Notebook"

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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
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  <img id="ntbook_pic" src="https://static.igem.org/mediawiki/2015/f/fe/Uppsala_notebook.png">
 
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   <h1>Notebook</h1>
 
   <h1>Notebook</h1>
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<h4>Week 1: (8-14 June)</h4>
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  <p><ul><li>Primers were designed for PCR extraction of the naphthalene upper pathway of the Nah7 plasmid from Pseudomonas putida, and to remove T7-promoters from CueO, CotA and catechol 1,2-dioxygenase biobricks.  We also designed primers to do overlap extension PCR to remove the scar that was created when attaching the HlyA-tag with the CueO, CotA and catechol 1,2-dioxygenase genes.</li></ul>
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Revision as of 23:59, 18 September 2015


Notebook

Week 1: (8-14 June)

  • Primers were designed for PCR extraction of the naphthalene upper pathway of the Nah7 plasmid from Pseudomonas putida, and to remove T7-promoters from CueO, CotA and catechol 1,2-dioxygenase biobricks. We also designed primers to do overlap extension PCR to remove the scar that was created when attaching the HlyA-tag with the CueO, CotA and catechol 1,2-dioxygenase genes.