Difference between revisions of "Team:Chalmers-Gothenburg/Basic Part"
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− | <h2> | + | <h2>BioBricks</h2> |
+ | <h3>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h3> | ||
+ | <p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in </i>S.cerevisiae</i>.</p> | ||
− | < | + | <h3>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h3> |
− | + | <p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p> | |
− | + | ||
− | </ | + | |
− | < | + | <h3>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h3> |
− | + | <p>Promoter to TPI1.</p> | |
− | </p> | + | |
− | <p> | + | <h3>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h3> |
+ | <p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms. | ||
+ | Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> | ||
− | </ | + | <h3>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h3> |
− | + | <p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> | |
− | </ | + |
Latest revision as of 00:07, 19 September 2015
![iGEM-Chalmers logo](https://static.igem.org/mediawiki/2015/5/5c/Chalmers_logo.png)
![Team Chalmers Gothenburg - A study in scarlet](https://static.igem.org/mediawiki/2015/8/84/Study_in_scarlet.png)
Contents
- 1 BioBricks
- 1.1 1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2
- 1.2 2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2
- 1.3 3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1
- 1.4 4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA
- 1.5 5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB
BioBricks
1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2
Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (Schizosaccharomyces pombe) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from S.pombe through the Pheromone pathway in </i>S.cerevisiae</i>.
2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2
The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.
3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1
Promoter to TPI1.
4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA
Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.
5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB
Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.