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| Our team developed a <a href="https://2015.igem.org/Team:Minnesota/Modeling">Mathematical Model </a> to estimate gene order for optimal biosynthetic production using 2A sequences and gladly present it as an open-source community tool to streamline future applications. We also attempted to demonstrate the utility of this technology in yeast by expressing genes to produce compounds in the beta carotenoid pathway. | | Our team developed a <a href="https://2015.igem.org/Team:Minnesota/Modeling">Mathematical Model </a> to estimate gene order for optimal biosynthetic production using 2A sequences and gladly present it as an open-source community tool to streamline future applications. We also attempted to demonstrate the utility of this technology in yeast by expressing genes to produce compounds in the beta carotenoid pathway. |
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| (4) Emmerman, M.; Temin, H. M., Comparison of promoter suppression in avian and murine retrovirus vectors. Nucleic Acids Res. 1986, 14(23): 9381-9396 | | (4) Emmerman, M.; Temin, H. M., Comparison of promoter suppression in avian and murine retrovirus vectors. Nucleic Acids Res. 1986, 14(23): 9381-9396 |
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− | (5) Geier, M.; Fauland, P.; Vogl, T.; Glieder, A., Compact multi-enzyme pathways in P. pastoris. Chem. Commun. 2014, 51, 1643-1646. | + | (5) Geier, M.; Fauland, P.; Vogl, T.; Glieder, A., Compact multi-enzyme pathways in P. pastoris. Chem. Commun. 2014, 51, 1643- |
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− | (6) Keasling, J. D.; Mendoza, A.; Baran, P. S., Synthesis: A constructive debate. Nature. 2012, 492: 188-189
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− | (7) Ryan, M. D.; Drew, J., Foot-and-mouth disease virus 2A oligopeptide mediated cleabage of an artificial polyprotein. EMBO J. 1994, 13(4): 928-933
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− | (8) Zhu, T.; Guo, M.; Sun, C.; Qian, J.; Zhuang, Y.; Chu, J.; Zhang S., A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains. Biotechnol Lett. 2009, 31(5): 679-84
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