Difference between revisions of "Team:NAIT Edmonton/Modeling"
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<center><h1>How Does SDS PAGE Work?</h1></center> | <center><h1>How Does SDS PAGE Work?</h1></center> | ||
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+ | <h3>SDS PAGE is a technique used to separate proteins by their molecular weight. Within a sample, there are multiple different types of protein. If we wanted to study a specific one, we would have to separate the different types first and isolate our desired protein. </h3> <br> | ||
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+ | <h3>As a quick overview, proteins are first denatured and linearized by SDS after being suspended in a protein sample loading buffer. Additionally, the SDS coats proteins with a negative charge. Immediately after denaturation, proteins are loaded into the wells of a polyacrylamide gel and an electric current is passed through it. The negatively charged proteins are pulled through the gel and migrate towards the electrode.</h3> | ||
Revision as of 00:52, 19 September 2015
Modeling