Difference between revisions of "Team:BIOSINT Mexico/Achivements"
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<br>The Figure 6 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. Likewise on the line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.</br> | <br>The Figure 6 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. Likewise on the line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.</br> | ||
| − | <br><img class="responsive-img" src="https://static.igem.org/mediawiki/2015/9/97/GELBIOSINT2015.png" width="60%" style="position: center; top:0%;"/><p>Figure 6: Agarose gel with spisPink and amigGFP samples, optimized for E. coli K12 (x3)</p></br> | + | <br></div><img class="responsive-img" src="https://static.igem.org/mediawiki/2015/9/97/GELBIOSINT2015.png" width="60%" style="position: center; top:0%;"/><p>Figure 6: Agarose gel with spisPink and amigGFP samples, optimized for E. coli K12 (x3)</p></div></br> |
</h5> | </h5> | ||
<h3>RBS and Optimized biobricks</h3> | <h3>RBS and Optimized biobricks</h3> | ||
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<br>The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 3 and Line 4 contain duplicate products of BBa_K1684002 (BBa_B0034 + BBa_K1684000) biobrick, both results match the size of the plasmids, 2800 bp. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material. </br> | <br>The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 3 and Line 4 contain duplicate products of BBa_K1684002 (BBa_B0034 + BBa_K1684000) biobrick, both results match the size of the plasmids, 2800 bp. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material. </br> | ||
| − | <br><img class="responsive-img" src="https://static.igem.org/mediawiki/2015/5/50/GEL1BIOSINT2015.jpeg" width="20%" style="position: center; top:0%;"/><p>Figure 7: Agarose gel with BBa_K1684002 (x2) and BBa_K1684003(x2) samples, positive and negative controls</p></br> | + | <br></div><img class="responsive-img" src="https://static.igem.org/mediawiki/2015/5/50/GEL1BIOSINT2015.jpeg" width="20%" style="position: center; top:0%;"/><p>Figure 7: Agarose gel with BBa_K1684002 (x2) and BBa_K1684003(x2) samples, positive and negative controls</p></div></br> |
</h5> | </h5> | ||
<h3>Optimized biobricks with Terminator</h3> | <h3>Optimized biobricks with Terminator</h3> | ||
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<br>In Line 3 and 4 results, was run the composite BioBrick BBa_K1684005, in the first case the result was not successful; In Line 4 however, the band is present with the respective size, 2890 bp, as a positive result. Finally on Line 5 is presented only the negative control.</br> | <br>In Line 3 and 4 results, was run the composite BioBrick BBa_K1684005, in the first case the result was not successful; In Line 4 however, the band is present with the respective size, 2890 bp, as a positive result. Finally on Line 5 is presented only the negative control.</br> | ||
| − | <br><img class="responsive-img" src="https://static.igem.org/mediawiki/2015/b/b9/GEL3BIOSINT2015.png | + | <br></div><img class="responsive-img" src="https://static.igem.org/mediawiki/2015/b/b9/GEL3BIOSINT2015.png |
| − | " width="15%" style="position: center; top:0%;"/><p>Figure 8: Agarose gel with BBa_K1684004 (x2) and BBa_K1684005(x2) samples, and negative control.</p></br> | + | " width="15%" style="position: center; top:0%;"/><p>Figure 8: Agarose gel with BBa_K1684004 (x2) and BBa_K1684005(x2) samples, and negative control.</p></div></br> |
</h5> | </h5> | ||
</div> | </div> | ||
Revision as of 00:55, 19 September 2015
Results
spisPink & amilGFP optimized to Escherichia coli K12
After completing the insertion of gBlocks (IDT) designed (spisPink and amilGFP), into the plasmid pSB1C3, by following the relevant protocols; competent cells were successfully transformed and consequently the removal of plasmids (spisPink into pSB1C3) BBa_K1684000 and (amilGFP into pSB1C3) BBa_K1684001 was performed.
The Figure 6 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. Likewise on the line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.
Figure 6: Agarose gel with spisPink and amigGFP samples, optimized for E. coli K12 (x3)
RBS and Optimized biobricks
In Figure 7 different samples of parts used for transformation of competent cells; these two parts are composite biobricks, the first is composed by RBS (BBa_B0034) and the optimized chromoprotein sequence spisPink for E. coli K12 (BBa_K1684000), while the second is composed by a RBS (BBa_B0034) and the optimized chromoprotein sequence amilGFP (BBa_K1684001 ).
The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 3 and Line 4 contain duplicate products of BBa_K1684002 (BBa_B0034 + BBa_K1684000) biobrick, both results match the size of the plasmids, 2800 bp. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material.
Figure 7: Agarose gel with BBa_K1684002 (x2) and BBa_K1684003(x2) samples, positive and negative controls
