Difference between revisions of "Team:Uppsala/Parts"
| Line 243: | Line 243: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688000</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688000">BBa_K1688000</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>J23101 + B0034 + RhlA + B0034 + RhlB</td> | + | <td>2343 bp (J23101 + B0034 + RhlA + B0034 + RhlB)</td> |
<td></td> | <td></td> | ||
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688001</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688001">BBa_K1688001</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>B0034 + RhlA + B0034 + RhlB</td> | + | <td>2300 bp (B0034 + RhlA + B0034 + RhlB)</td> |
<td></td> | <td></td> | ||
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688002</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688002">BBa_K1688002</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>B0034 + RhlA</td> | + | <td>965 bp(B0034 + RhlA)</td> |
<td></td> | <td></td> | ||
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688003</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688003">BBa_K1688003</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>B0034 + RhlB</td> | + | <td>1327 bp (B0034 + RhlB)</td> |
<td></td> | <td></td> | ||
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688004</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688004">BBa_K1688004</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>726 bp</td> |
<td>An RBS assembled with the dTomato coding sequence, expressing a modified RFP.</td> | <td>An RBS assembled with the dTomato coding sequence, expressing a modified RFP.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688005</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688005">BBa_K1688005</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>3810 bp</td> |
<td>The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase.</td> | <td>The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688006</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688006">BBa_K1688006</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>1800 bp</td> |
| − | <td></td> | + | <td>A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688007</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688007">BBa_K1688007</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>1843 bp</td> |
<td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag.</td> | <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688008</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688008">BBa_K1688008</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>1651 bp</td> |
<td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag.</td> | <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688009</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688009">BBa_K1688009</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>4977 bp</td> |
<td>The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase.</td> | <td>The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688010</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688010">BBa_K1688010</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>769 bp</td> |
<td>A constitutive Anderson promoter assembled with the dTomato reporter gene.</td> | <td>A constitutive Anderson promoter assembled with the dTomato reporter gene.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688011</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688011">BBa_K1688011</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>769 bp</td> |
<td>A constitutive Anderson promoter assembled with the dTomato reporter gene.</td> | <td>A constitutive Anderson promoter assembled with the dTomato reporter gene.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688012</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688012">BBa_K1688012</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>2002 bp</td> |
<td>The NahR construct assembled with the dTomato reporter gene.</td> | <td>The NahR construct assembled with the dTomato reporter gene.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688013</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688013">BBa_K1688013</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>9487 bp</td> |
<td>The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS</td> | <td>The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688014</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688014">BBa_K1688014</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>9487 bp</td> |
<td>The naphthalene pathway with the BBa_J23101 promoter and the B0034 RBS</td> | <td>The naphthalene pathway with the BBa_J23101 promoter and the B0034 RBS</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688017</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688017">BBa_K1688017</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>9444 bp</td> |
<td>The naphthalene pathway, with the B0034 RBS and both PstI sites mutagenized</td> | <td>The naphthalene pathway, with the B0034 RBS and both PstI sites mutagenized</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K1688015</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1688015">BBa_K1688015</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td>9444 bp</td> |
<td>The naphthalene pathway, with the B0034 RBS.</td> | <td>The naphthalene pathway, with the B0034 RBS.</td> | ||
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
Revision as of 01:13, 19 September 2015
Parts
| Biobrick Code | Type | Length | Description | Designers |
|---|---|---|---|---|
| BBa_K1688000 | Coding | 2343 bp (J23101 + B0034 + RhlA + B0034 + RhlB) | Biosurfers | |
| BBa_K1688001 | Coding | 2300 bp (B0034 + RhlA + B0034 + RhlB) | Biosurfers | |
| BBa_K1688002 | Coding | 965 bp(B0034 + RhlA) | Biosurfers | |
| BBa_K1688003 | Coding | 1327 bp (B0034 + RhlB) | Biosurfers | |
| BBa_K1688004 | Coding | 726 bp | An RBS assembled with the dTomato coding sequence, expressing a modified RFP. | Laccalicious |
| BBa_K1688005 | Coding | 3810 bp | The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase. | Laccalicious |
| BBa_K1688006 | Coding | 1800 bp | A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus. | Laccalicious |
| BBa_K1688007 | Coding | 1843 bp | A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag. | Laccalicious |
| BBa_K1688008 | Coding | 1651 bp | A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag. | Laccalicious |
| BBa_K1688009 | Coding | 4977 bp | The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase. | Laccalicious |
| BBa_K1688010 | Coding | 769 bp | A constitutive Anderson promoter assembled with the dTomato reporter gene. | Laccalicious |
| BBa_K1688011 | Coding | 769 bp | A constitutive Anderson promoter assembled with the dTomato reporter gene. | Laccalicious |
| BBa_K1688012 | Coding | 2002 bp | The NahR construct assembled with the dTomato reporter gene. | Laccalicious |
| BBa_K1688013 | Coding | 9487 bp | The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS | Laccalicious |
| BBa_K1688014 | Coding | 9487 bp | The naphthalene pathway with the BBa_J23101 promoter and the B0034 RBS | Laccalicious |
| BBa_K1688017 | Coding | 9444 bp | The naphthalene pathway, with the B0034 RBS and both PstI sites mutagenized | Laccalicious |
| BBa_K1688015 | Coding | 9444 bp | The naphthalene pathway, with the B0034 RBS. | Laccalicious |