Difference between revisions of "Team:Tec-Monterrey/Notebook"
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Latest revision as of 01:21, 19 September 2015
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Miniprep (Dania, Hiyam) Digestion (Carolina) Electrophoresis (David, Alex) |
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Stock RFP (Dania) |
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Miniprep (Hiyam, Dania) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Ausencio, Dania, Hiyam) |
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Miniprep (Ausencio, Dania, Hiyam) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Karen Carrizales, Dania) |
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Calcium-competent cells (Jesús) |
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Transformation (David, Ernesto) |
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Inoculations (Alejandro, Carolina, Jesús, Dania) |
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Miniprep (David, Alejandro) Digestion (Alejandro, Hiyam) Electrophoresis (Alejandro, Ernesto) Digestion (Alejandro, David, Ernesto) Electrophoresis (David, Ernesto) |
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
|---|---|---|---|---|---|---|
1
Purification (David, Minerva, Ernesto) Digestions (Carolina, Karen Carrizales) Electrophoresis (Carolina, Dania, Ausencio) Ligations (Jesús, Alex, Hiyam) |
2
Purification (Carolina, Alejandro) Ligations (Carolina, Alejandro) Transformation (David, Karen Carrizales) |
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Stock P4 (Hiyam) Miniprep (Hiyam, Carolina) |
4
Digestion (Hiyam, David) Electrophoresis (Carolina) Transformation (David, Jesús) Digestion (Karen Carrizales, Alejandro) Electrophoresis (Alejandro, Karen Carrizales, David) Purification (Hiyam, Dania) Ligations (Hiyam, Alejandro, Dania) |
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Transformation (Jesús, Carolina) Inoculations (David, Dania, Karen Carrizales, Alejandro) |
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Stocks P1, P2, P3, GFP (Carolina, Dania) Miniprep (Carolina, Dania, Ausencio) Inoculations (Jesus, Hiyam) |
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Degestions (Carolina, Dania, Hiyam) Miniprep (Hiyam, Jesús, David, Ausencio) Digestions (Ausencio, Dania, Hiyam) |
8
Electrophoresis (David, Hiyam, Karen Carrizales, Dania) Purification (Karen Carrizales, Ausencio, David) Electrophoresis (Hiyam, Dania) Ligations (David, Karen Carrizales, Ernesto) Transformation (Minerva) |
9
Electrophoresis (David, Ausencio, Ernesto) Digestion (David, Ernesto) Electrophoresis (David, Carolina, Dania) Purification (Hiyam, Dania) Ligations (David) Miniprep Lowe Budget (Alejandra, Karen Tello) |
10
Digestion (Carolina) Electrophoresis (David, Ausencio) Transformation (David, Ausencio) Purification (Ausencio, David, Dania, Karen Carrizales) Digestion Low Budget (Alejandra, Karen Tello) |
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Ligations (Carolina) |
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Transformation (Carolina, Jesús, Ausencio) |
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Inoculations (Carolina, David, Karen Carrizales) |
14
Miniprep (Carolina, Ausencio, David) Digestion (Hiyam, David) Transformation (Karen Carrizales, David) Electrophoresis (Hiyam, David) |
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Inoculations (Karen Carrizales, Jesús) |
16
Miniprep (David, Hiyam) Digestion (David, Hiyam) Electrophoresis (Carolina, Karen Carrizales) Purification (Hiyam, Karen Carrizales, David) |
17
Antibiotic preparation (Hiyam, Carolina) Transformation (Karen Carrizales, David) |
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Miniprep (David) Digestion (Karen Carrizales, Ausencio) Electrophoresis, Purification (Hiyam, David) Inoculations (Ausencio) |
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Miniprep (David, Hiyam) Miniprep Low Budget, Digestion Lowe Budget (Alejandra, Karen Tello) |
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Digestion (Hiyam, Alejandra, Claudia) Electrophoresis (Karen Carrizales, Hiyam) Miniprep (David) Digestion (Hiyam, David) Electrophoresis (Hiyam) |
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Calcium-competent cells (Claudia) |
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24
Miniprep (Hiyam, Dania, Carolina) Calcium-competent cells (Claudia) Transformation, Stocks P1 P2 P3 TOP10 DH5alpha (Alejandro) Digestion (Hiyam, Caro, Dania) Electrophoresis (David, Karen Carrizales) |
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Miniprep (Dania, Hiyam, Caro) Digestion, Electrophoresis (David, Alejandro) Digestion (Carolina, Alejandro) Sf9 cells counting and passing (Minerva) |
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Digestion (Carolina, David, Hiyam) Electrophoresis (Hiyam, Alejandro) Transformation (Alejandro) Sf9 cells counting and passing (Dania, Claudia) |
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Digestion (Hiyam, Dania, David) Electrophoresis (David, Hiyam, Carolina) Purification (Alejandro, Hiyam) Digestion (Alejandro, Hiyam) Electrophoresis (Hiyam, Alejandro, David) |
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Electrophoresis (Alejandro, David) Purification (Carolina, David) Digestion (Carolina, Alejandro, Hiyam, Dania) Electrophoresis (David) |
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
|---|---|---|---|---|---|---|
1
Purification (Hiyam, Dania) Ligation (Hiyam, Alex, Dania) Inoculations (Dania, Hiyam, Alex, David) Transformation (Carolina, Hiyam, Alejandro) |
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2
Digestion, Electrophoresis (Alejandro) Calcium-competent cells (Minerva, David) Transformation (Alejandro, Hiyam) Sf9 cells counting and passing (Dania, Claudia) |
3
Stocks C2, C4, C5, P4, pFastBac (Alejandro, Hiyam) Miniprep (David, Hiyam) Digestion (Hiyam, David, Dania) Calcium-competent cells (Jesús, Karen Carrizales), Electrophoresis (Carolina, Dania) |
4
Digestion, Electrophoresis (Alejandro, Karen Carrizales, David) |
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Cell Lysis (Alejandro) Transformation (Hiyam, Alejandro, Karen Tello) |
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Ligation, Digestion (Hiyam, Alejandro) Miniprep (Hiyam) |
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Miniprep 1 (David) Miniprep 2 (Alejandro, Karen Tello) Digestion (Karen Tello, Lourdes, Jesús) Electrophoresis (Karen Tello, Lourdes) Sf9 cells counting, passing and criopreservation (Dania, Claudia) |
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Electrophoresis (Alejandro) Inoculations (Jesús, David) |
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Miniprep 1 (Carolina, Karen Carrizales) Miniprep 2 (David) Miniprep 3 (Karen Tello, Alejandro, Hiyam) Digestion (Hiyam) Bacmid extraction (David) Sf9 cells counting and passing (Dania, Claudia) |
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Electrophoresis, Miniprep (Hiyam) Electrophoresis (Karen Tello, Ausencio) Inoculations (Carolina) Transfection (David, Dania, Minerva) |
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Miniprep (Carolina) Transformation (Alejandro) |
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SDS-Page (David) Western Blot (Minerva, David) |
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Luminiscence readings, Biuret Assay (Alejandro) Sf9 cells counting and passing (Dania, Claudia) |
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Sf9 cells counting and passing (Dania, Claudia) SDS-Page (David) Western Blot (David, Minerva) Luminiscence readings (Minerva, Alejandro) Virus amplification (Jesús, Karen Carrizales, Claudia) |
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Calcium-competents (Alejandro, Hiyam, Ausencio) Transformation, Digestion, Electrophoresis (Hiyam, Karen Tello, Ausencio) Purification, Ligation (Hiyam, Carolina) Inoculations, Miniprep (Carolina, Karen Tello) |
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Sf9 cells counting and passing (Dania, Claudia) |
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Sf9 cells counting and passing (Dania, Claudia) |
23
Minprep (David) Digestion (Carolina) Bacmid extraction (Karen Carrizales, Carolina) Electrophoresis (Carolina) Inoculations (Karen Tello, Carolina) Sf9 cells counting and passing (Dania, Claudia) |
24
Transfection (David, Dania, Minerva) |
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Miniprep, Digestion, Electrophoresis, Gibson Assembly (Hiyam, Karen Tello, Ausencio) Bradford (Alejandro) Sf9 cells counting and passing (Dania, Claudia) |
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Digestion (Alejandro) |
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Miniprep, Digestion, Electrophoresis (Hiyam, Alejandro, Ausencio) Purification (Eduardo, Hiyam, Alejandro, Ausencio) |
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Gibson Assembly (Eduardo, Alejandro, Hiyam, Ausencio) Sf9 cells counting and passing (Dania, Claudia) |
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Viral plaque assay (Jesús, Karen Carrizales, Claudia) |
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Sf9 cells counting and passing (Dania, Claudia) |
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
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Sf9 cells counting and passing (Dania, Claudia) |
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3 |
4
Sf9 cells counting and passing (Dania, Claudia) |
5
Digestion (Hiyam) Sf9 cells counting and passing (Dania, Claudia) |
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Sf9 cells counting and passing (Dania, Claudia) Viral plaque assay (Jesús, Karen Carrizales, Claudia) |
7
Gibson Assembly (Hiyam) Sf9 cells counting and passing (Dania, Claudia) |
8
Sf9 cells counting and passing (Dania, Claudia) |
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Gibson Assembly (Eduardo, Hiyam, Alejandro, Ausencio) Sf9 cells counting and passing (Dania, Claudia) |
10
Sf9 cells counting and passing (Dania, Claudia) Transfection (David, Dania, Minerva) |
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Sf9 cells counting and passing (Dania, Claudia) |
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Sf9 cells counting and passing (Dania, Claudia |
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Viral plaque staining, viral plaque assay (Karen Carrizales, Jesús) |
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Digestion (Alejandro, Ausencio) Electrophoresis (David, Eduardo, Ausencio) IMAC chromatography (Karen Carrizales, Jesús, Alejandro) SDS-Page (David) Western Blot (David, Minerva) |
16
Miniprep (Alejandro, Hiyam) SDS-Page, Western Blot (David, Minerva) |
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Confirmation of parts for submitting (Karen Tello, Ausencio, Hiyam, Alejandro, Eduardo) |
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