Difference between revisions of "Team:NUDT CHINA/Notebook"

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<style type="text/css">
 
<style type="text/css">
 
#bannerContainer {
 
#bannerContainer {
−
margin-left:180px;
+
margin: 0px 180px;
−
margin-right:180px;
+
padding-top: 30px;
 +
padding-bottom: 0px;
 
width: auto;
 
width: auto;
 
}
 
}
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clear: both;
 
clear: both;
 
width: auto;
 
width: auto;
−
margin: 30px 180px;
+
margin: 0px 180px;
−
padding: 30px 0px;
+
padding: 20px 0px 30px 0px;
 
font-family: 'verdana',sans-serif;
 
font-family: 'verdana',sans-serif;
 
background: #fff;
 
background: #fff;
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{
 
{
 
width: auto;
 
width: auto;
 +
}
 +
</style>
 +
<!--NUDT_Footer-->
 +
<style type="text/css">
 +
#NUDT_Footer
 +
{
 +
clear: both;
 +
width: auto;
 +
padding: 30px 0px;
 +
font-family: 'verdana',sans-serif;
 +
position: relative;
 +
}
 +
#NUDT_Footer p{
 +
text-decoration: none;
 +
font-family: "Trebuchet MS", Helvetica, sans-serif;
 +
color: #000000;
 +
position: relative;
 +
padding: 10px 50px;
 +
font-size: 16px;
 
}
 
}
 
</style>
 
</style>
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<li><a id = "Basic" href = "https://2015.igem.org/Team:NUDT_CHINA/Basic_Part" class = "b"><span>Basic Parts</span></a></li>
 
<li><a id = "Basic" href = "https://2015.igem.org/Team:NUDT_CHINA/Basic_Part" class = "b"><span>Basic Parts</span></a></li>
 
<li><a id = "Composite" href="https://2015.igem.org/Team:NUDT_CHINA/Composite_Part"  class = "b"><span>Composite Parts</span></a></li>
 
<li><a id = "Composite" href="https://2015.igem.org/Team:NUDT_CHINA/Composite_Part"  class = "b"><span>Composite Parts</span></a></li>
 +
<li><a id = "Composite" href="https://2015.igem.org/Team:NUDT_CHINA/Part_Collection"  class = "b"><span>Part Collection</span></a></li>
  
  
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<li><a id = "SAFETY" href="https://2015.igem.org/Team:NUDT_CHINA/Safety"><span>SAFETY</span></a></li>  
 
<li><a id = "SAFETY" href="https://2015.igem.org/Team:NUDT_CHINA/Safety"><span>SAFETY</span></a></li>  
 
<li><a id = "MODELING" href="https://2015.igem.org/Team:NUDT_CHINA/Modeling"><span>MODELING</span></a></li>   
 
<li><a id = "MODELING" href="https://2015.igem.org/Team:NUDT_CHINA/Modeling"><span>MODELING</span></a></li>   
−
<li><a id = "MEASUREMENT" href="https://2015.igem.org/Team:NUDT_CHINA/Measurement"><span>MEASUREMENT</span></a></li> 
 
−
<li><a id = "ENTREPRENEURSHIP" href="https://2015.igem.org/Team:NUDT_CHINA/Entrepreneurship"><span>ENTREPRENEURSHIP</span></a></li>
 
 
    </div>
 
    </div>
 
    <div id = "logoContainer">
 
    <div id = "logoContainer">
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<section id="cd-timeline" class="cd-container">
 
<section id="cd-timeline" class="cd-container">
 
<!--2015.5.16-->
 
<!--2015.5.16-->
 +
<p id = "pos5"></p>
 
<div class="cd-timeline-block">
 
<div class="cd-timeline-block">
 
<div class="cd-timeline-img cd-picture" id = "pic1">
 
<div class="cd-timeline-img cd-picture" id = "pic1">
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<div class="cd-timeline-content" id = "key1">
 
<div class="cd-timeline-content" id = "key1">
 
<h7 style = "color:black"><h7 style = "color: #d82545">CULTURE&amp;SQUENCE:</h7> Related parts in Kit</h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545">CULTURE&amp;SQUENCE:</h7> Related parts in Kit</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont1" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<p style = "padding: 10px 5px;" id = "cont1" class = "Pannal">After long time waiting and discussing, we can start our experience now.</p>
 
<span class="cd-date" style="font-size:24px">2015.5.16</span>
 
<span class="cd-date" style="font-size:24px">2015.5.16</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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<!--2015.5.31-->
 
<!--2015.5.31-->
 
<div class="cd-timeline-block">
 
<div class="cd-timeline-block">
−
<p id = "pos5"></p>
 
 
<div class="cd-timeline-img cd-picture" id = "pic2">
 
<div class="cd-timeline-img cd-picture" id = "pic2">
 
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
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<div class="cd-timeline-content" id = "key2">
 
<div class="cd-timeline-content" id = "key2">
 
<h7 style = "color:black"><h7 style = "color: #d82545"> ACTIVITY: </h7>Move the lab.</h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545"> ACTIVITY: </h7>Move the lab.</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont2" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<p style = "padding: 10px 5px;" id = "cont2" class = "Pannal">Because of various restrictions, we move our lat to an idle lab.</p>
 
<span class="cd-date" style="font-size:24px">2015.5.31</span>
 
<span class="cd-date" style="font-size:24px">2015.5.31</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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<div class="cd-timeline-content" id = "key3">
 
<div class="cd-timeline-content" id = "key3">
 
<h7 style = "color:black"><h7 style = "color: #d82545">PCR:</h7>  IaaM&amp;IaaH </h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545">PCR:</h7>  IaaM&amp;IaaH </h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont3" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
 
 +
<div id = "cont3" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/2/25/NUDT_CHINA-Notebook1.png"></center>
 +
<p style = "padding: 10px 5px;">We got fragments of IAAM &amp; IAAH to do their standardization next.</p></div>
 +
 
 
<span class="cd-date" style="font-size:24px">2015.6.7</span>
 
<span class="cd-date" style="font-size:24px">2015.6.7</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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</div>
 
</div>
 
</div>
 
</div>
−
<!--2015.6.26-->
+
<!--2015.6.14-->
 
<div class="cd-timeline-block">
 
<div class="cd-timeline-block">
−
<div class="cd-timeline-img cd-picture" id = "pic4">
+
<p id = "pos17"></p>
 +
<div class="cd-timeline-img cd-picture" id = "pic17">
 
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
</div>
 
</div>
−
<div class="cd-timeline-content" id = "key4">
+
<div class="cd-timeline-content" id = "key17">
−
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> IaaM, SCAF1, TALE2</h7>
+
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE:</h7> IaaM&amp;IaaH </h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont4" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
 
−
<span class="cd-date" style="font-size:24px">2015.6.26</span>
+
<div id = "cont17" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/b/b8/NUDT_CHINA-Notebook2.png"></center>
 +
<p style = "padding: 10px 5px;">At this point, we could use them convenient.</p></div>
 +
 
 +
<span class="cd-date" style="font-size:24px">2015.6.14</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
 
$(document).ready(function(){
 
$(document).ready(function(){
−
$("#key4").click(function(){
+
$("#key17").click(function(){
−
$("#cont4").slideToggle(300);
+
$("#cont17").slideToggle(300);
 
});
 
});
 
});
 
});
 
$(document).ready(function(){
 
$(document).ready(function(){
−
$("#pic4").click(function(){
+
$("#pic17").click(function(){
−
$("#cont4").slideToggle(300);
+
$("#cont17").slideToggle(300);
 +
});
 +
});
 +
</script>
 +
</div>
 +
</div>
 +
<!--2015.6.21-->
 +
<div class="cd-timeline-block">
 +
<p id = "pos18"></p>
 +
<div class="cd-timeline-img cd-picture" id = "pic18">
 +
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 +
</div>
 +
<div class="cd-timeline-content" id = "key18">
 +
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE:</h7>  TALE1 </h7>
 +
 
 +
<div id = "cont18" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/a/a7/NUDT_CHINA-Notebook3.png"></center>
 +
<p style = "padding: 10px 5px;">This fragment came from NJU_CHINA, we standardized it ourselves.</p></div>
 +
 
 +
<span class="cd-date" style="font-size:24px">2015.6.21</span>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$("#key18").click(function(){
 +
$("#cont18").slideToggle(300);
 +
});
 +
});
 +
$(document).ready(function(){
 +
$("#pic18").click(function(){
 +
$("#cont18").slideToggle(300);
 
});
 
});
 
});
 
});
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</div>
 
</div>
 
<div class="cd-timeline-content" id = "key5">
 
<div class="cd-timeline-content" id = "key5">
−
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> SCAF2,SCAF3,TALE1,TALE3</h7>
+
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> SCAF1, SCAF2, SCAF3, TALE2, TALE3</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont5" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont5" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/7/7b/NUDT_CHINA-Notebook4.png"></center>
 +
<p style = "padding: 10px 5px;">We got SCAF1, SCAF2, SCAF3 a few days ago, and followed the NJU_CHINA produced TALE2,TALE3. Nowadays we standardize them.</p></div>
 
<span class="cd-date" style="font-size:24px">2015.6.28</span>
 
<span class="cd-date" style="font-size:24px">2015.6.28</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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<div class="cd-timeline-content" id = "key6">
 
<div class="cd-timeline-content" id = "key6">
 
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> Plac+RBS30</h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> Plac+RBS30</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont6" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont6" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/3/39/NUDT_CHINA-Notebook5.png"></center>
 +
<p style = "padding: 10px 5px;">Plac+RBS30 is an useful fragment, we standardized it in order to use it.</p></div>
 
<span class="cd-date" style="font-size:24px">2015.6.29</span>
 
<span class="cd-date" style="font-size:24px">2015.6.29</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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$("#pic6").click(function(){
 
$("#pic6").click(function(){
 
$("#cont6").slideToggle(300);
 
$("#cont6").slideToggle(300);
−
});
 
−
});
 
−
</script>
 
−
</div>
 
−
</div>
 
−
<!--2015.6.29-->
 
−
<div class="cd-timeline-block">
 
−
<div class="cd-timeline-img cd-picture" id = "pic7">
 
−
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
−
</div>
 
−
<div class="cd-timeline-content" id = "key7">
 
−
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD:</h7> Plac+RBS30+TALE1</h7>
 
−
<p style = "padding: 10px 5px; display:none;" id = "cont7" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
 
−
<span class="cd-date" style="font-size:24px">2015.6.29</span>
 
−
<script type="text/javascript">
 
−
$(document).ready(function(){
 
−
$("#key7").click(function(){
 
−
$("#cont7").slideToggle(300);
 
−
});
 
−
});
 
−
$(document).ready(function(){
 
−
$("#pic7").click(function(){
 
−
$("#cont7").slideToggle(300);
 
−
});
 
−
});
 
−
</script>
 
−
</div>
 
−
</div>
 
−
<!--2015.7.5-->
 
−
<div class="cd-timeline-block">
 
−
<p id = "pos7"></p>
 
−
<div class="cd-timeline-img cd-picture" id = "pic8">
 
−
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
−
</div>
 
−
<div class="cd-timeline-content" id = "key8">
 
−
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD:</h7> Adding terminator&amp;RBS to GFP1 by PCR</h7>
 
−
<p style = "padding: 10px 5px; display:none;" id = "cont8" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
 
−
<span class="cd-date" style="font-size:24px">2015.7.5</span>
 
−
<script type="text/javascript">
 
−
$(document).ready(function(){
 
−
$("#key8").click(function(){
 
−
$("#cont8").slideToggle(300);
 
−
});
 
−
});
 
−
$(document).ready(function(){
 
−
$("#pic8").click(function(){
 
−
$("#cont8").slideToggle(300);
 
−
});
 
−
});
 
−
</script>
 
−
</div>
 
−
</div>
 
−
<!--2015.7.24-->
 
−
<div class="cd-timeline-block">
 
−
<div class="cd-timeline-img cd-picture" id = "pic9">
 
−
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
−
</div>
 
−
<div class="cd-timeline-content" id = "key9">
 
−
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> Iaa H</h7>
 
−
<p style = "padding: 10px 5px; display:none;" id = "cont9" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
 
−
<span class="cd-date" style="font-size:24px">2015.7.24</span>
 
−
<script type="text/javascript">
 
−
$(document).ready(function(){
 
−
$("#key9").click(function(){
 
−
$("#cont9").slideToggle(300);
 
−
});
 
−
});
 
−
$(document).ready(function(){
 
−
$("#pic9").click(function(){
 
−
$("#cont9").slideToggle(300);
 
 
});
 
});
 
});
 
});
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<!--2015.7.25-->
 
<!--2015.7.25-->
 
<div class="cd-timeline-block">
 
<div class="cd-timeline-block">
 +
<p id = "pos7"></p>
 
<div class="cd-timeline-img cd-picture" id = "pic10">
 
<div class="cd-timeline-img cd-picture" id = "pic10">
 
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 
</div>
 
</div>
 
<div class="cd-timeline-content" id = "key10">
 
<div class="cd-timeline-content" id = "key10">
−
<h7 style = "color:black"><h7 style = "color: #d82545">Device(No.1): </h7> GFP(+/-)N</h7>
+
<h7 style = "color:black"><h7 style = "color: #d82545">Device(No.1): </h7> GFP–N1</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont10" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont10" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/b/bc/NUDT_CHINA-Notebook6.png"></center>
 +
<p style = "padding: 10px 5px;">We finished the first device. This device is one of negative control for GFP.</p></div>
 
<span class="cd-date" style="font-size:24px">2015.7.25</span>
 
<span class="cd-date" style="font-size:24px">2015.7.25</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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</div>
 
</div>
 
<div class="cd-timeline-content" id = "key11">
 
<div class="cd-timeline-content" id = "key11">
−
<h7 style = "color:black"><h7 style = "color: #d82545">SYSTEM(No.1): </h7> GFP(+/-)S1</h7>
+
<h7 style = "color:black"><h7 style = "color: #d82545">Device(No.2): </h7> GFP-S1</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont11" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont11" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/5/58/NUDT_CHINA-Notebook7.png"></center>
 +
<p style = "padding: 10px 5px;">We finished the second device which is the experimental group.</p></div>
 
<span class="cd-date" style="font-size:24px">2015.7.27</span>
 
<span class="cd-date" style="font-size:24px">2015.7.27</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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</div>
 
</div>
 
<div class="cd-timeline-content" id = "key14">
 
<div class="cd-timeline-content" id = "key14">
−
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7>GFP(+/+)N</h7>
+
<h7 style = "color:black"><h7 style = "color: #d82545">Device(No.3): </h7>GFP-N2</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont14" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont14" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/1/16/NUDT_CHINA-Notebook8.png"></center>
 +
<p style = "padding: 10px 5px;">This device is also one of negative control for GFP.</p></div>
 
<span class="cd-date" style="font-size:24px">2015.7.31</span>
 
<span class="cd-date" style="font-size:24px">2015.7.31</span>
 
<script type="text/javascript">
 
<script type="text/javascript">
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<div class="cd-timeline-content" id = "key12">
 
<div class="cd-timeline-content" id = "key12">
 
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> RBS30</h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545">STANDARDIZAE: </h7> RBS30</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont12" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont12" class = "Pannal">
 +
<p style = "padding: 10px 5px;">The RBS30 in kit is mistake. We rework it by annealing.</p></div>
 
<span class="cd-date" style="font-size:24px">2015.8.12</span>
 
<span class="cd-date" style="font-size:24px">2015.8.12</span>
 
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<div class="cd-timeline-content" id = "key13">
 
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7>IAA(+/+)N</h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7>IAA(+/+)N</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont13" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont13" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/5/56/NUDT_CHINA-Notebook9.png"></center></div>
 
<span class="cd-date" style="font-size:24px">2015.8.17</span>
 
<span class="cd-date" style="font-size:24px">2015.8.17</span>
 
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<div class="cd-timeline-content" id = "key15">
 
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7> IAA(+/+)S2/S3, GFP P</h7>
 
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7> IAA(+/+)S2/S3, GFP P</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont15" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont15" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/c/c5/NUDT_CHINA-Notebook10.png"></center></div>
 
<span class="cd-date" style="font-size:24px">2015.8.19</span>
 
<span class="cd-date" style="font-size:24px">2015.8.19</span>
 
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<div class="cd-timeline-content" id = "key16">
−
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7>GFP(+/+)S2/S3</h7>
+
<h7 style = "color:black"><h7 style = "color: #d82545">BUILD: </h7>GFP-S2, GFP-S3, GFP-P</h7>
−
<p style = "padding: 10px 5px; display:none;" id = "cont16" class = "Pannal"><img src="test1.png">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut justo nibh, mattis sit amet consequat a, varius vitae metus. Proin pharetra sodales pellentesque.</p>
+
<div id = "cont16" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/5/5d/NUDT_CHINA-Notebook11.png"></center></div>
 
<span class="cd-date" style="font-size:24px">2015.8.20</span>
 
<span class="cd-date" style="font-size:24px">2015.8.20</span>
 
<script type="text/javascript">
 
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</div>
 
</div>
 
</div>
 +
<!--2015.8.26—2015.9.6-->
 +
<div class="cd-timeline-block">
 +
<p id = "pos9"></p>
 +
<div class="cd-timeline-img cd-picture" id = "pic20">
 +
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 +
</div>
 +
<div class="cd-timeline-content" id = "key20">
 +
<h7 style = "color:black"><h7 style = "color: #d82545">REBUILD: </h7>GFP-S1, GFP-S2, GFP-S3, GFP-N1, GFP-N2, IAA, IAA-S2, IAA-S3.</h7>
 +
<div id = "cont20" class = "Pannal">
 +
<p style = "padding: 10px 5px;">From the result of DNA sequencing, we found the GFP2 from kits had 1 bp too many. Because of this, GFP2 expression is abnormal. We rebuild the GFP2 by PCR, and rebuild the devices. Also from the result of DNA sequencing, the RBS30 in IAA, IAA-S2 and IAA-S3 were disappeared. We rebuild them too.</p></div>
 +
<span class="cd-date" style="font-size:24px">2015.8.26 — 2015.9.6</span>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$("#key20").click(function(){
 +
$("#cont20").slideToggle(300);
 +
});
 +
});
 +
$(document).ready(function(){
 +
$("#pic20").click(function(){
 +
$("#cont20").slideToggle(300);
 +
});
 +
});
 +
</script>
 +
</div>
 +
</div>
 +
<div class="cd-timeline-block">
 +
<div class="cd-timeline-img cd-picture" id = "pic21">
 +
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 +
</div>
 +
<div class="cd-timeline-content" id = "key21">
 +
<h7 style = "color:black"><h7 style = "color: #d82545">ChIP: </h7>GFP series</h7>
 +
<div id = "cont21" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/9/96/NUDT_CHINA-Notebook12.png"></center>
 +
<p style = "padding: 10px 5px;">This experiment proved the function of TALE.</p></div>
 +
<span class="cd-date" style="font-size:24px">2015.9.4 - 2015.9.6</span>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$("#key21").click(function(){
 +
$("#cont21").slideToggle(300);
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});
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$(document).ready(function(){
 +
$("#pic21").click(function(){
 +
$("#cont21").slideToggle(300);
 +
});
 +
});
 +
</script>
 +
</div>
 +
</div>
 +
<div class="cd-timeline-block">
 +
<div class="cd-timeline-img cd-picture" id = "pic22">
 +
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 +
</div>
 +
<div class="cd-timeline-content" id = "key22">
 +
<h7 style = "color:black"><h7 style = "color: #d82545">ELISA: </h7>IAA, IAA-S2, IAA-S3</h7>
 +
<div id = "cont22" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/9/95/NUDT_CHINA-Notebook13.png"></center>
 +
<p style = "padding: 10px 5px;">We found that the production for auxin increased when there have SCAF. And the close two enzymes got, the higher efficiency can be.</p></div>
 +
<span class="cd-date" style="font-size:24px">2015.9.8</span>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$("#key22").click(function(){
 +
$("#cont22").slideToggle(300);
 +
});
 +
});
 +
$(document).ready(function(){
 +
$("#pic22").click(function(){
 +
$("#cont22").slideToggle(300);
 +
});
 +
});
 +
</script>
 +
</div>
 +
</div>
 +
<div class="cd-timeline-block">
 +
<div class="cd-timeline-img cd-picture" id = "pic23">
 +
<img src="https://static.igem.org/mediawiki/2015/9/98/Smile_NUDT_CHINA.png" alt="Picture">
 +
</div>
 +
<div class="cd-timeline-content" id = "key23">
 +
<h7 style = "color:black"><h7 style = "color: #d82545">RT: </h7>IAA, IAA-S2, IAA-S3</h7>
 +
<div id = "cont22" class = "Pannal">
 +
<center><img src="https://static.igem.org/mediawiki/2015/7/7a/NUDT_CHINA-Notebook14.png"></center>
 +
<p style = "padding: 10px 5px;">We could see that the production for RNA got close.</p></div>
 +
<span class="cd-date" style="font-size:24px">2015.9.9</span>
 +
<script type="text/javascript">
 +
$(document).ready(function(){
 +
$("#key23").click(function(){
 +
$("#cont23").slideToggle(300);
 +
});
 +
});
 +
$(document).ready(function(){
 +
$("#pic22").click(function(){
 +
$("#cont23").slideToggle(300);
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});
 +
});
 +
</script>
 +
</div>
 +
</div>
 +
  
 
</div>
 
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<div id = "navigation">
 
<div id = "navigation">
 
<img src="https://static.igem.org/mediawiki/2015/5/58/TEAM_NUDT_CHINA_iGEM.png"/>
 
<img src="https://static.igem.org/mediawiki/2015/5/58/TEAM_NUDT_CHINA_iGEM.png"/>
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<li><a href="#myMainContainer" class = "t"><span>TOP</span></a></li>
+
<li><a href="#myMainContainer" class = "t"><span>TOP/TIP</span></a></li>
 
<li><a href = "#pos5"><span>2015.5</span></a></li>
 
<li><a href = "#pos5"><span>2015.5</span></a></li>
 
<li><a href = "#pos6"><span>2015.6</span></a></li>
 
<li><a href = "#pos6"><span>2015.6</span></a></li>
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<img src="https://static.igem.org/mediawiki/2015/9/93/Team_NUDT_CHINA_Navigate.png"/>
 
<img src="https://static.igem.org/mediawiki/2015/9/93/Team_NUDT_CHINA_Navigate.png"/>
 
    </div>
 
    </div>
 +
<div id = "NUDT_Footer">
 +
<center>
 +
<p>Copyright © 2015 NUDT_CHINA | Powered by Nianhao Xie</p>
 +
</center>
 +
</div>
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 01:38, 19 September 2015

NOTEBOOK

TIPS1: The left MENU :) links to other NUDT_CHINA website.

TIPS2: The right NAVIGATE :) posites on content in this page. (Valid when FOLDED/UNFOLDED)

TIPS3: Click SUBTITLE/:) to FOLD/UNFOLD related content.

Picture
CULTURE&SQUENCE: Related parts in Kit

After long time waiting and discussing, we can start our experience now.

2015.5.16
Picture
ACTIVITY: Move the lab.

Because of various restrictions, we move our lat to an idle lab.

2015.5.31

Picture
PCR: IaaM&IaaH

We got fragments of IAAM & IAAH to do their standardization next.

2015.6.7

Picture
STANDARDIZAE: IaaM&IaaH

At this point, we could use them convenient.

2015.6.14

Picture
STANDARDIZAE: TALE1

This fragment came from NJU_CHINA, we standardized it ourselves.

2015.6.21
Picture
STANDARDIZAE: SCAF1, SCAF2, SCAF3, TALE2, TALE3

We got SCAF1, SCAF2, SCAF3 a few days ago, and followed the NJU_CHINA produced TALE2,TALE3. Nowadays we standardize them.

2015.6.28
Picture
STANDARDIZAE: Plac+RBS30

Plac+RBS30 is an useful fragment, we standardized it in order to use it.

2015.6.29

Picture
Device(No.1): GFP–N1

We finished the first device. This device is one of negative control for GFP.

2015.7.25
Picture
Device(No.2): GFP-S1

We finished the second device which is the experimental group.

2015.7.27
Picture
Device(No.3): GFP-N2

This device is also one of negative control for GFP.

2015.7.31

Picture
STANDARDIZAE: RBS30

The RBS30 in kit is mistake. We rework it by annealing.

2015.8.12
Picture
BUILD: IAA(+/+)N
2015.8.17
Picture
BUILD: IAA(+/+)S2/S3, GFP P
2015.8.19
Picture
BUILD: GFP-S2, GFP-S3, GFP-P
2015.8.20

Picture
REBUILD: GFP-S1, GFP-S2, GFP-S3, GFP-N1, GFP-N2, IAA, IAA-S2, IAA-S3.

From the result of DNA sequencing, we found the GFP2 from kits had 1 bp too many. Because of this, GFP2 expression is abnormal. We rebuild the GFP2 by PCR, and rebuild the devices. Also from the result of DNA sequencing, the RBS30 in IAA, IAA-S2 and IAA-S3 were disappeared. We rebuild them too.

2015.8.26 — 2015.9.6
Picture
ChIP: GFP series

This experiment proved the function of TALE.

2015.9.4 - 2015.9.6
Picture
ELISA: IAA, IAA-S2, IAA-S3

We found that the production for auxin increased when there have SCAF. And the close two enzymes got, the higher efficiency can be.

2015.9.8
Picture
RT: IAA, IAA-S2, IAA-S3

We could see that the production for RNA got close.

2015.9.9