Difference between revisions of "Team:Northeastern Boston/Description"

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<p>We then shifted towards adaption of a plasmid from the Chlamy Collection: pOpt_mVenus. By surrounding the first promoter and first intron with the iGEM prefix and suffix, we created an iGEM compatible protein expression <a href="https://2015.igem.org/Team:Northeastern_Boston/Design" target="_blank">plasmid</a>. In this way, teams can remove the suffix and replace it with a codon-optimized coding sequence for heterologous proteins of interest, or remove the promoter region entirely, testing alternate promoters and coding sequences upstream of a hygromycin B selection cassette.</p>
 
<p>We then shifted towards adaption of a plasmid from the Chlamy Collection: pOpt_mVenus. By surrounding the first promoter and first intron with the iGEM prefix and suffix, we created an iGEM compatible protein expression <a href="https://2015.igem.org/Team:Northeastern_Boston/Design" target="_blank">plasmid</a>. In this way, teams can remove the suffix and replace it with a codon-optimized coding sequence for heterologous proteins of interest, or remove the promoter region entirely, testing alternate promoters and coding sequences upstream of a hygromycin B selection cassette.</p>
  
<p>Genetic engineering of microalgae is not new. <i>C. reinhardtii</i>, in particular, has been explored as a platform for heterologous proteins for years, although at far lesser levels than mammalian cells or higher-order plants. Being a nearly ideal chassis, <i>C. reinhardtii</i> represents the organism of the future.</p>
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<p>Genetic engineering of microalgae is not new. <i>C. reinhardtii</i>, in particular, has been explored as a platform for heterologous proteins for years, although at far lesser levels than mammalian cells or higher-order plants. Although it's more difficult to work with than bacteria, and less throughly explored than yeast, microalgae represent is poised to disrupt in areas like biofuel, agriculture, and pharmaceuticals. Being a nearly ideal chassis, <i>C. reinhardtii</i> represents the organism of the future.</p>
  
 
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Revision as of 01:39, 19 September 2015

Overview

The Need

Some Solutions

A Green Safety Net

Cost/Benefit

Approach