Difference between revisions of "Team:William and Mary/Notebook"
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− | < | + | <h1>June</h1> |
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<div class="separator line-separator">✻</div> | <div class="separator line-separator">✻</div> | ||
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− | <h3>Week 1 ( | + | <h3>Week 1 (6/1-6/7)</h3> |
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<li>Resuspended all parts from the kit</li> | <li>Resuspended all parts from the kit</li> | ||
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<li>Began performing Gibson PCRs</li> | <li>Began performing Gibson PCRs</li> | ||
</ul> | </ul> | ||
− | <h3>Week 2 ( | + | <h3>Week 2 (6/8-6/14)</h3> |
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<li>Troubleshot Gibson PCRs</li> | <li>Troubleshot Gibson PCRs</li> | ||
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<li>Designed Gibson assemblies to put together different fluorescent proteins, each under the control of the same promoter</li> | <li>Designed Gibson assemblies to put together different fluorescent proteins, each under the control of the same promoter</li> | ||
<li>Attempted to Gibson assemble YFP + CFP under control of R0010</li></ul> | <li>Attempted to Gibson assemble YFP + CFP under control of R0010</li></ul> | ||
− | <h3>Week 3 ( | + | <h3>Week 3 (6/15-6/21)</h3> |
<ul> | <ul> | ||
<li>Re-attempted Gibson assembly to make a double fluorescent construct with proteins under R0010</li> | <li>Re-attempted Gibson assembly to make a double fluorescent construct with proteins under R0010</li> | ||
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<li>Began assembling different promoters with our fluorescent constructs (to eventually collect noise data for each promoter)</li> | <li>Began assembling different promoters with our fluorescent constructs (to eventually collect noise data for each promoter)</li> | ||
<li>Designed and started the assembly process to make KanR (necessary to integrate parts onto the E. coli genome)</li></ul> | <li>Designed and started the assembly process to make KanR (necessary to integrate parts onto the E. coli genome)</li></ul> | ||
− | <h3>Week 4 ( | + | <h3>Week 4 (6/22-6/28)</h3> |
<ul><li>Many of our past assemblies (dCas9, double fluorescent constructs, etc) failed. We continued attempts at these assemblies.</li></ul> | <ul><li>Many of our past assemblies (dCas9, double fluorescent constructs, etc) failed. We continued attempts at these assemblies.</li></ul> | ||
</p> | </p> | ||
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− | < | + | <h1>July</h1> |
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− | <h3 | + | <h3>Week 1 (6/29-7/5)</h3> |
− | + | <ul><li>We have assembled our gRNAs and dCas9!</li> | |
− | We have assembled our gRNAs and dCas9! | + | <li>We ordered primers to integrate onto the E. coli genome</li> |
− | We ordered primers to integrate onto the E. coli genome | + | <li>Designed assembly to put a KanR operon after a fluorescent construct to integrate</li> |
− | Designed assembly to put a KanR operon after a fluorescent construct to integrate | + | <li>Designed a better way to assemble double fluorescent constructs</li> |
− | Designed a better way to assemble double fluorescent constructs | + | <li>Designed a way to make a TetR operon to integrate two different things</li> |
− | Designed a way to make a TetR operon to integrate two different things | + | <li>Made electrocompetent cells containing our dCas9 operon</li> |
− | Made electrocompetent cells containing our dCas9 operon | + | <li>Assembled a double fluorescent construct! CFP and YFP under R0010</li> |
− | Assembled a double fluorescent construct! CFP and YFP under R0010 | + | <li>Attempted our first double electroporation-transformation to test our dCas9 and gRNAs</li></ul> |
− | Attempted our first double electroporation-transformation to test our dCas9 and gRNAs | + | <h3>Week 2 (7/6-7/12)</h3> |
− | Week 2 (7/6-7/12) | + | <ul><li>Began assembling CFP and YFP operons with each promoter that we wanted to test</li> |
− | Began assembling CFP and YFP operons with each promoter that we wanted to test | + | <li>Confirmed that dCas9 and our gRNA design works</li> |
− | Confirmed that dCas9 and our gRNA design works | + | <li>Assembled the CFP under R0010 with KanR, so that it can be PCR’d with the integration PCRs, and integrated</li> |
− | Assembled the CFP under R0010 with KanR, so that it can be PCR’d with the integration PCRs, and integrated | + | <li>Attempted to make TetR operon</li></li> |
− | Attempted to make TetR operon | + | <h3>Week 3 (7/13-7/19)</h3> |
− | Week 3 (7/13-7/19) | + | <ul><li>Attempted to test our integration protocol, by integrating KanR onto the E. coli genome</li> |
− | Attempted to test our integration protocol, by integrating KanR onto the E. coli genome | + | <li>Continued attempting to construct Interlab Parts</li> |
− | Continued attempting to construct Interlab Parts | + | <li>Worked on our collaboration with UGA</li> |
− | Worked on our collaboration with UGA | + | <li>Designed gRNAs for other promoters to create a suite of repression parts</li> |
− | Designed gRNAs for other promoters to create a suite of repression parts | + | <li>Continued assembling CFP and YFP operons with promoters of interest</li> |
− | Continued assembling CFP and YFP operons with promoters of interest | + | <li>Continued attempts to make TetR operon</li></ul> |
− | Continued attempts to make TetR operon | + | <h3>Week 4 (7/20-7/26)</h3> |
− | Week 4 (7/20-7/26) | + | <ul><li>Began testing integrator cassette part</li> |
− | Began testing integrator cassette part | + | <li>Continued working on Interlab Study</li> |
− | Continued working on Interlab Study | + | <li>Continued assembling CFP and YFP operons</li> |
− | Continued assembling CFP and YFP operons | + | <li>Continued attempts to make TetR operon</li></ul> |
− | Continued attempts to make TetR operon | + | <h3>Week 5 (7/26-8/2)</h3> |
− | Week 5 (7/26-8/2) | + | <ul><li>Ordered gBlocks for Interlab Study</li> |
− | Ordered gBlocks for Interlab Study | + | <li>Continued working on assembling all gRNA parts</li> |
− | Continued working on assembling all gRNA parts | + | <li>Continued assembling CFP and YFP operons</li> |
− | Continued assembling CFP and YFP operons | + | <li>Imaged our single integrated fluorescent CFP (under R0010)</li> |
− | Imaged our single integrated fluorescent CFP (under R0010) | + | <li>Continued attempts to make TetR operon</li></ul> |
− | Continued attempts to make TetR operon | + | |
</p> | </p> | ||
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Revision as of 01:57, 19 September 2015
Our Notebook On this page you can view the work we did each month, from June through September. You can also find our protocols. Important Protocols Easy access to our most complex protocols. Improved own provided blessing may peculiar domestic. Sight house has sex never. No visited raising gravity outward subject my cottage mr be. Hold do at tore in park feet near my case. Invitation at understood occasional sentiments insipidity inhabiting in. Off melancholy alteration principles old. Is do speedily kindness properly oh. Respect article painted cottage he is offices parlors. Week 1 (8/3-8/9)
Continued assembling CFP and YFP operons
Began assembling CFP operons with KanR (to integrate)
Continued attempts to make TetR operon
8/10-8/23: School mandates that we leave
During this time, we learned that TetR had been mislabelled, and the part we were actually looking to assemble to confer tetracycline resistance was “TetA”
We designed PCRs to assemble TetA
Week 4 (8/24-8/30)
Continued working on Interlab Study
Began assembling YFP operons with TetA (to integrate)
Began attempts to integrate YFP operons with TetA for which there was a corresponding CFP operon with KanR
Each Pencil is milled from a single, solid piece of material. Graphite brushed aluminum model shown. Pencil’s certified Bluetooth Smart wireless delivers a fast, stable connection with industry-leading power conservation Each Pencil is milled from a single, solid piece of material. Graphite brushed aluminum model shown.June
Week 1 (6/1-6/7)
Week 2 (6/8-6/14)
Week 3 (6/15-6/21)
Week 4 (6/22-6/28)
July
Week 1 (6/29-7/5)
Week 2 (7/6-7/12)
Week 3 (7/13-7/19)
Week 4 (7/20-7/26)
Week 5 (7/26-8/2)
Project Content
Example No.3