Difference between revisions of "Team:uOttawa/Description"
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+ | <h1>Improvement</h1> | ||
+ | <p>Our biobrick, <a href="http://parts.igem.org/Part:BBa_K1652000">pPGK1Gx</a>, is an improvement to <a href="http://parts.igem.org/Part:BBa_K1400000">pTRE(4)Gx</a> and <a href="http://parts.igem.org/Part:BBa_K1400001">pTRE(2)Gx</a> from uOttawa's 2014 project. While the previous parts were GEV-repressible but required rtTA to express, pPGK1Gx has strong, constitutive expression. This part does not require the cell to have rtTA to get a signal, and does not require the user to maintain a steady concentration of anhydrotetracycline or doxycycline to modulate the rtTA activity. This way, rtTA is free to be used as a transcription factor elsewhere in a genetic network.</p> | ||
+ | <p>Not only does this behaviour fit with achieving tri-stability, but it is also ideal for implementing new logic gates with genes. This promoter, being repressible, can be used as a single-input NOT gate with a twist. Its input may be able to induce a <i>positive</i> signal somewhere else.</p> | ||
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p> | <p>Tell us about your project, describe what moves you and why this is something important for your team.</p> | ||
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Revision as of 02:04, 19 September 2015
Improvement
Our biobrick, pPGK1Gx, is an improvement to pTRE(4)Gx and pTRE(2)Gx from uOttawa's 2014 project. While the previous parts were GEV-repressible but required rtTA to express, pPGK1Gx has strong, constitutive expression. This part does not require the cell to have rtTA to get a signal, and does not require the user to maintain a steady concentration of anhydrotetracycline or doxycycline to modulate the rtTA activity. This way, rtTA is free to be used as a transcription factor elsewhere in a genetic network.
Not only does this behaviour fit with achieving tri-stability, but it is also ideal for implementing new logic gates with genes. This promoter, being repressible, can be used as a single-input NOT gate with a twist. Its input may be able to induce a positive signal somewhere else.