Difference between revisions of "Team:NJAU China/Parts"
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<div class="section"> | <div class="section"> | ||
+ | |||
+ | <table border="1" cellspacing="0" cellpadding="0"> | ||
+ | <tr> | ||
+ | <td width="61" valign="top"><p>Label</p></td> | ||
+ | <td width="103" valign="top"><p>Name</p></td> | ||
+ | <td width="75" valign="top"><p>Type</p></td> | ||
+ | <td width="277" valign="top"><p>Description</p></td> | ||
+ | <td width="53" valign="top"><p>Designer</p></td> | ||
+ | <td width="77" valign="top"><p>Length</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="61" valign="top"><p> </p></td> | ||
+ | <td width="103" valign="top"><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649000">BBa_K1649000</a></p></td> | ||
+ | <td width="75" valign="top"><p>Composite</p></td> | ||
+ | <td width="277" valign="top"><p>red light sensor</p></td> | ||
+ | <td width="53" valign="top"><p>Yang Yang</p></td> | ||
+ | <td width="77" valign="top"><p>4000bp</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="61" valign="top"><p> </p></td> | ||
+ | <td width="103" valign="top"><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649001">BBa_K1649001</a></p></td> | ||
+ | <td width="75" valign="top"><p>Composite</p></td> | ||
+ | <td width="277" valign="top"><p>red light sensor with a GFP reporter </p></td> | ||
+ | <td width="53" valign="top"><p>Yang Yang</p></td> | ||
+ | <td width="77" valign="top"><p>4883bp</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="61" valign="top"><p><img border="0" width="24" height="22" src="file:///F|/igem/clip_image002.gif"></p></td> | ||
+ | <td width="103" valign="top"><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649002">BBa_K1649002</a></p></td> | ||
+ | <td width="75" valign="top"><p>Composite</p></td> | ||
+ | <td width="277" valign="top"><p>Red light sensor with TetR not gate and GF<a name="_GoBack"></a>P reporter </p></td> | ||
+ | <td width="53" valign="top"><p>Yang Yang</p></td> | ||
+ | <td width="77" valign="top"><p>7280bp</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="61" valign="top"><p><img border="0" width="24" height="22" src="file:///F|/igem/clip_image002_0000.gif"></p></td> | ||
+ | <td width="103" valign="top"><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649003">BBa_K1649003</a></p></td> | ||
+ | <td width="75" valign="top"><p>Composite</p></td> | ||
+ | <td width="277" valign="top"><p>YF1 blue light sensor with GFP reporter </p></td> | ||
+ | <td width="53" valign="top"><p>Yang Yang</p></td> | ||
+ | <td width="77" valign="top"><p>3117bp</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="61" valign="top"><p> </p></td> | ||
+ | <td width="103" valign="top"><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649004">BBa_K1649004</a><u> </u></p></td> | ||
+ | <td width="75" valign="top"><p>Composite</p></td> | ||
+ | <td width="277" valign="top"><p>YF1-FixJ with terminator </p></td> | ||
+ | <td width="53" valign="top"><p>Yang Yang</p></td> | ||
+ | <td width="77" valign="top"><p>1933bp</p></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<h2>BBa_K1649000</h2> | <h2>BBa_K1649000</h2> | ||
<p>Red Light Sensor</p> | <p>Red Light Sensor</p> |
Revision as of 02:39, 19 September 2015
Label |
Name |
Type |
Description |
Designer |
Length |
|
Composite |
red light sensor |
Yang Yang |
4000bp |
|
|
Composite |
red light sensor with a GFP reporter |
Yang Yang |
4883bp |
|
Composite |
Yang Yang |
7280bp |
|||
Composite |
YF1 blue light sensor with GFP reporter |
Yang Yang |
3117bp |
||
|
Composite |
YF1-FixJ with terminator |
Yang Yang |
1933bp |
BBa_K1649000
Red Light Sensor
he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.
BBa_K1649001
Red Light Sensor with a GFP Reporter
FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.
BBa_K1649002
Red Light Sensor with TetR Not Gate and GFP Reporter
s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.
BBa_K1649004
YF1-FixJ with Terminator
he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.
BBa_K1649003
YF1 Blue Light Sensor with GFP Reporter
FP is used to report the function of blue light sensor.