Difference between revisions of "Template:Heidelberg/project/standardization/onthesouthern"

 
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                                     Not only can the HRP be used in the ApatBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation<x-ref>Kershaw2012</x-ref> (Fig. 4). This system can be used to avoid working with radioactive isotopes and also poses an alternative to using fluorescent probes.
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                                     Not only can the HRP be used in the AptaBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation<x-ref>Kershaw2012</x-ref> (Fig. 4). This system can be used to avoid working with radioactive isotopes and also poses an alternative to using fluorescent probes.
  
 
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RNA and DNA could be label with HRP-mimicking DNAzyme via splinted ligation
 
RNA and DNA could be label with HRP-mimicking DNAzyme via splinted ligation
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Figure 5. Switchable HRP DNAzyme for software validation
 
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HRP-mimicking DNAzyme activity is controlled by an aptamer that stems with part of the active site in absence of the ligand. In presence of the ligand the inhibiting stem is destabled and thus the HRP-mimicking DNAzyme's activity is recovered.
 
 
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Latest revision as of 02:41, 19 September 2015

On the Southern Blot

Not only can the HRP be used in the AptaBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligationKershaw2012 (Fig. 4). This system can be used to avoid working with radioactive isotopes and also poses an alternative to using fluorescent probes.

Figure 4. DNA or RNA labeled with HRP-mimicking DNAzyme

RNA and DNA could be label with HRP-mimicking DNAzyme via splinted ligation