Difference between revisions of "Team:NJAU China/Parts"
Cassie0707 (Talk | contribs) |
Cassie0707 (Talk | contribs) |
||
Line 24: | Line 24: | ||
<div class="section"> | <div class="section"> | ||
− | <table | + | <table> |
<tr> | <tr> | ||
− | <td | + | <td><p>Label</p></td> |
− | <td | + | <td><p>Name</p></td> |
− | <td | + | <td><p>Type</p></td> |
− | <td | + | <td><p>Description</p></td> |
− | <td | + | <td><p>Designer</p></td> |
− | <td | + | <td><p>Length</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td | + | <td><p> </p></td> |
− | <td | + | <td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649000">BBa_K1649000</a></p></td> |
− | <td | + | <td><p>Composite</p></td> |
− | <td | + | <td><p>red light sensor</p></td> |
− | <td | + | <td><p>Yang Yang</p></td> |
− | <td | + | <td><p>4000bp</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td | + | <td><p> </p></td> |
− | <td | + | <td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649001">BBa_K1649001</a></p></td> |
− | <td | + | <td><p>Composite</p></td> |
− | <td | + | <td><p>red light sensor with a GFP reporter </p></td> |
− | <td | + | <td><p>Yang Yang</p></td> |
− | <td | + | <td><p>4883bp</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td | + | <td><p><img border="0" width="24" height="22" src="file:///F|/igem/clip_image002.gif"></p></td> |
− | <td | + | <td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649002">BBa_K1649002</a></p></td> |
− | <td | + | <td><p>Composite</p></td> |
− | <td | + | <td><p>Red light sensor with TetR not gate and GF<a name="_GoBack"></a>P reporter </p></td> |
− | <td | + | <td><p>Yang Yang</p></td> |
− | <td | + | <td><p>7280bp</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td | + | <td><p><img border="0" width="24" height="22" src="file:///F|/igem/clip_image002_0000.gif"></p></td> |
− | <td | + | <td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649003">BBa_K1649003</a></p></td> |
− | <td | + | <td><p>Composite</p></td> |
− | <td | + | <td><p>YF1 blue light sensor with GFP reporter </p></td> |
− | <td | + | <td><p>Yang Yang</p></td> |
− | <td | + | <td><p>3117bp</p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td | + | <td><p> </p></td> |
− | <td | + | <td><p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1649004">BBa_K1649004</a><u> </u></p></td> |
− | <td | + | <td ><p>Composite</p></td> |
− | <td | + | <td><p>YF1-FixJ with terminator </p></td> |
− | <td | + | <td><p>Yang Yang</p></td> |
− | <td | + | <td><p>1933bp</p></td> |
</tr> | </tr> | ||
</table> | </table> |
Revision as of 02:55, 19 September 2015
![](https://static.igem.org/mediawiki/2015/f/fc/NJAU_China2015_teamparts.jpg)
Label |
Name |
Type |
Description |
Designer |
Length |
|
Composite |
red light sensor |
Yang Yang |
4000bp |
|
|
Composite |
red light sensor with a GFP reporter |
Yang Yang |
4883bp |
|
Composite |
Yang Yang |
7280bp |
|||
Composite |
YF1 blue light sensor with GFP reporter |
Yang Yang |
3117bp |
||
|
Composite |
YF1-FixJ with terminator |
Yang Yang |
1933bp |
BBa_K1649000
Red Light Sensor
he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.
![](https://static.igem.org/mediawiki/2015/f/f0/NJAU_2015_teamparts1.png)
BBa_K1649001
Red Light Sensor with a GFP Reporter
FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.
![](https://static.igem.org/mediawiki/2015/6/64/NJAU_2015_teamparts2.png)
BBa_K1649002
Red Light Sensor with TetR Not Gate and GFP Reporter
s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.
![](https://static.igem.org/mediawiki/2015/1/11/NJAU_2015_teamparts3.png)
BBa_K1649004
YF1-FixJ with Terminator
he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.
![](https://static.igem.org/mediawiki/2015/0/04/NJAU_2015_teamparts4.png)
BBa_K1649003
YF1 Blue Light Sensor with GFP Reporter
FP is used to report the function of blue light sensor.
![](https://static.igem.org/mediawiki/2015/9/9b/NJAU_2015_teamparts5.png)