Difference between revisions of "Team:York/Protocols"

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<h2>Lysogeny Broth</h2>
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<h3>Materials</h3>
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<ul>
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<li>10g of tryptone</li>
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<li>5g of yeast extract</li>
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<li>10g of NaCl</li>
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<li>1L of Deionized Water</li>
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<li> 1M NaCl </li>
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<li> 1M KOH </li>
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</ul>
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<h3>Procedure</h3>
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<ol>
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<li>Use a container with at least double the volume of the liquid that you are making.</li>
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<li>Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water. </li>
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<li>Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter. </li>
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<li>Autoclave. </li>
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<li>Store at room temperature or +4°C.</li>
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</ol>

Revision as of 14:01, 9 July 2015

Protocols

Lysogeny Broth

Materials

  • 10g of tryptone
  • 5g of yeast extract
  • 10g of NaCl
  • 1L of Deionized Water
  • 1M NaCl
  • 1M KOH

Procedure

  1. Use a container with at least double the volume of the liquid that you are making.
  2. Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
  3. Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
  4. Autoclave.
  5. Store at room temperature or +4°C.