Difference between revisions of "Team:SZMS 15 Shenzhen/Experiments"
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<div class="heading-text-title">Experiment</div> | <div class="heading-text-title">Experiment</div> | ||
| − | <div class="heading-text-content"> In this magical world with advanced technology, the classic stories and fables become a little different | + | <div class="heading-text-content"> In this magical world with advanced technology, the classic stories and fables become a little different. |
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<div class="content1-text-content"> We use Biobricks to connect parts together. The restriction enzyme cutting sites are Ecor1, Xba1, Spe1 and Pst1. | <div class="content1-text-content"> We use Biobricks to connect parts together. The restriction enzyme cutting sites are Ecor1, Xba1, Spe1 and Pst1. | ||
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| + | <div class="content1-text-title">PCR mutagenesis | ||
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| + | <div class="content1-text-content"> In common scenarios, lacI repressor will create leak expression, causing the expression of Plac even without the presence of lactose. However, according to an article, they claim to have found several kinds of “super repressor” with no leak expression. The article has given the gene sequence of these types of “super repressor”, we have chosen one and use PCR conducted site-specific mutagenesis to engineer the part P0412 in order to better achieve the system. | ||
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Revision as of 03:20, 19 September 2015
Experiment
In this magical world with advanced technology, the classic stories and fables become a little different.
Enzyme cut and synthesis
We use Biobricks to connect parts together. The restriction enzyme cutting sites are Ecor1, Xba1, Spe1 and Pst1.
PCR mutagenesis
In common scenarios, lacI repressor will create leak expression, causing the expression of Plac even without the presence of lactose. However, according to an article, they claim to have found several kinds of “super repressor” with no leak expression. The article has given the gene sequence of these types of “super repressor”, we have chosen one and use PCR conducted site-specific mutagenesis to engineer the part P0412 in order to better achieve the system.
"The ugly duckling"
In this world, green color is the symbol of purity, beauty and elegance. Our duckling is at first colorless, after it goes through the impediment of lactose, it turns green and becomes the swan.
"The Fox and the Grape"
In this world, grapes give out banana smell. The disgruntled fox not only just walk away and curse the grapes, it also stops the grape from giving out tantalizing smell!
"Houyi Shoots the Sun"
In this world, suns emit green color and continue rising temperature. Houyi can be controlled by us to shoot the suns. When shooting the sun, Houyi will emit red light passionately.
