Difference between revisions of "Team:Uppsala/Composite Part"
| Line 31: | Line 31: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23101 + B0034 + RhlA + B0034 + RhlB</td> | <td>J23101 + B0034 + RhlA + B0034 + RhlB</td> | ||
| − | <td></td> | + | <td>Mono-rhamnolipid genes (rhlA and rhlB) including RBS and J23101 promoter.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 38: | Line 38: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + RhlA + B0034 + RhlB</td> | <td>B0034 + RhlA + B0034 + RhlB</td> | ||
| − | <td></td> | + | <td>Mono-rhamnolipid genes (rhlA and rhlB) including RBS.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 45: | Line 45: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + RhlA</td> | <td>B0034 + RhlA</td> | ||
| − | <td></td> | + | <td>Mono-rhamnolipid gene rhlA including RBS.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 52: | Line 52: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + RhlB</td> | <td>B0034 + RhlB</td> | ||
| − | <td></td> | + | <td>Mono-rhamnolipid gene rhlB including RBS.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 59: | Line 59: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + dTomato</td> | <td>B0034 + dTomato</td> | ||
| − | <td></td> | + | <td>An RBS assembled with the dTomato coding sequence, expressing a modified RFP.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 66: | Line 66: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>NahR+ dTomato + ModLac</td> | <td>NahR+ dTomato + ModLac</td> | ||
| − | <td></td> | + | <td>The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 73: | Line 73: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23101 + ModLac with export tag</td> | <td>J23101 + ModLac with export tag</td> | ||
| − | <td></td> | + | <td>A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 80: | Line 80: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23110 + ModLac without export tag</td> | <td>J23110 + ModLac without export tag</td> | ||
| − | <td></td> | + | <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 87: | Line 87: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>NahR + dTomato + ModLac + Dioxygenase</td> | <td>NahR + dTomato + ModLac + Dioxygenase</td> | ||
| − | <td></td> | + | <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 94: | Line 94: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23101 + B0034 + dTomato</td> | <td>J23101 + B0034 + dTomato</td> | ||
| − | <td></td> | + | <td>The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 101: | Line 101: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23110 + B0034 + dTomato</td> | <td>J23110 + B0034 + dTomato</td> | ||
| − | <td></td> | + | <td>A constitutive Anderson promoter assembled with the dTomato reporter gene.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 108: | Line 108: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>NahR + dTomato</td> | <td>NahR + dTomato</td> | ||
| − | <td></td> | + | <td>The NahR construct assembled with the dTomato reporter gene.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
| Line 115: | Line 115: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23110+0034+napthtalene pathway</td> | <td>J23110+0034+napthtalene pathway</td> | ||
| − | <td></td> | + | <td>The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS.</td> |
<td>Nah7</td> | <td>Nah7</td> | ||
</tr> | </tr> | ||
| Line 122: | Line 122: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23101+0034+napthtalene pathway</td> | <td>J23101+0034+napthtalene pathway</td> | ||
| − | <td></td> | + | <td>The naphthalene pathway with the BBa_J23101 promoter and the B0034 RBS.</td> |
<td>Nah7</td> | <td>Nah7</td> | ||
</tr> | </tr> | ||
Revision as of 03:30, 19 September 2015
Composite Parts
| Biobrick Code | Type | Construct | Description | Designers |
|---|---|---|---|---|
| BBa_K1688000 | Coding | J23101 + B0034 + RhlA + B0034 + RhlB | Mono-rhamnolipid genes (rhlA and rhlB) including RBS and J23101 promoter. | Biosurfers |
| BBa_K1688001 | Coding | B0034 + RhlA + B0034 + RhlB | Mono-rhamnolipid genes (rhlA and rhlB) including RBS. | Biosurfers |
| BBa_K1688002 | Coding | B0034 + RhlA | Mono-rhamnolipid gene rhlA including RBS. | Biosurfers |
| BBa_K1688003 | Coding | B0034 + RhlB | Mono-rhamnolipid gene rhlB including RBS. | Biosurfers |
| BBa_K1688004 | Coding | B0034 + dTomato | An RBS assembled with the dTomato coding sequence, expressing a modified RFP. | Laccalicious |
| BBa_K1688005 | Coding | NahR+ dTomato + ModLac | The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase. | Laccalicious |
| BBa_K1688007 | Coding | J23101 + ModLac with export tag | A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus. | Laccalicious |
| BBa_K1688008 | Coding | J23110 + ModLac without export tag | A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag. | Laccalicious |
| BBa_K1688009 | Coding | NahR + dTomato + ModLac + Dioxygenase | A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag. | Laccalicious |
| BBa_K1688010 | Coding | J23101 + B0034 + dTomato | The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase. | Laccalicious |
| BBa_K1688011 | Coding | J23110 + B0034 + dTomato | A constitutive Anderson promoter assembled with the dTomato reporter gene. | Laccalicious |
| BBa_K1688012 | Coding | NahR + dTomato | The NahR construct assembled with the dTomato reporter gene. | Laccalicious |
| BBa_K1688013 | Coding | J23110+0034+napthtalene pathway | The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS. | Nah7 |
| BBa_K1688014 | Coding | J23101+0034+napthtalene pathway | The naphthalene pathway with the BBa_J23101 promoter and the B0034 RBS. | Nah7 |
| BBa_K1688015 | Coding | 0034+napthalene pathway 2*mutagenized | Nah7 | |
| BBa_K1688017 | Coding | 0034+napthalene pathway | Nah7 |