Difference between revisions of "Team:Uppsala/Composite Part"

Line 73: Line 73:
 
         <td>Coding</td>
 
         <td>Coding</td>
 
         <td>J23101 + ModLac with export tag</td>
 
         <td>J23101 + ModLac with export tag</td>
−
         <td>A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.</td>
+
         <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag.</td>
 
         <td>Laccalicious</td>
 
         <td>Laccalicious</td>
 
       </tr>
 
       </tr>
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         <td>Coding</td>
 
         <td>Coding</td>
 
         <td>J23110 + ModLac without export tag</td>
 
         <td>J23110 + ModLac without export tag</td>
−
         <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag.</td>
+
         <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag.</td>
 
         <td>Laccalicious</td>
 
         <td>Laccalicious</td>
 
       </tr>
 
       </tr>
Line 87: Line 87:
 
         <td>Coding</td>
 
         <td>Coding</td>
 
         <td>NahR + dTomato + ModLac + Dioxygenase</td>
 
         <td>NahR + dTomato + ModLac + Dioxygenase</td>
−
         <td>A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag.</td>
+
         <td>The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase.</td>
 
         <td>Laccalicious</td>
 
         <td>Laccalicious</td>
 
       </tr>
 
       </tr>
Line 94: Line 94:
 
         <td>Coding</td>
 
         <td>Coding</td>
 
         <td>J23101 + B0034 + dTomato</td>
 
         <td>J23101 + B0034 + dTomato</td>
−
         <td>The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase.</td>
+
         <td>A constitutive Anderson promoter assembled with the dTomato reporter gene.</td>
 
         <td>Laccalicious</td>
 
         <td>Laccalicious</td>
 
       </tr>
 
       </tr>

Revision as of 03:45, 19 September 2015

Composite Parts

Biobrick Code Type Construct Description Designers
BBa_K1688000 Coding J23101 + B0034 + RhlA + B0034 + RhlB Mono-rhamnolipid genes (rhlA and rhlB) including RBS and J23101 promoter. Biosurfers
BBa_K1688001 Coding B0034 + RhlA + B0034 + RhlB Mono-rhamnolipid genes (rhlA and rhlB) including RBS. Biosurfers
BBa_K1688002 Coding B0034 + RhlA Mono-rhamnolipid gene rhlA including RBS. Biosurfers
BBa_K1688003 Coding B0034 + RhlB Mono-rhamnolipid gene rhlB including RBS. Biosurfers
BBa_K1688004 Coding B0034 + dTomato An RBS assembled with the dTomato coding sequence, expressing a modified RFP. Laccalicious
BBa_K1688005 Coding NahR+ dTomato + ModLac The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase. Laccalicious
BBa_K1688007 Coding J23101 + ModLac with export tag A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag. Laccalicious
BBa_K1688008 Coding J23110 + ModLac without export tag A constitutive Anderson promoter assembled with the coding sequence for the modified laccase with an export tag. Laccalicious
BBa_K1688009 Coding NahR + dTomato + ModLac + Dioxygenase The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase. Laccalicious
BBa_K1688010 Coding J23101 + B0034 + dTomato A constitutive Anderson promoter assembled with the dTomato reporter gene. Laccalicious
BBa_K1688011 Coding J23110 + B0034 + dTomato A constitutive Anderson promoter assembled with the dTomato reporter gene. Laccalicious
BBa_K1688012 Coding NahR + dTomato The NahR construct assembled with the dTomato reporter gene. Laccalicious
BBa_K1688013 Coding J23110+0034+napthtalene pathway The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS. Nah7
BBa_K1688014 Coding J23101+0034+napthtalene pathway The naphthalene pathway with the BBa_J23101 promoter and the B0034 RBS. Nah7
BBa_K1688015 Coding 0034+napthalene pathway The naphthalene pathway, with the B0034 RBS. Nah7
BBa_K1688017 Coding 0034+napthalene pathway 2*mutagenized The naphthalene pathway, with the B0034 RBS and both PstI sites mutagenized. Nah7