Difference between revisions of "Team:KU Leuven/InterLabStudy/Results"

Table 1: The raw fluorescence data ( excitation at 483 nm and emission at 525 nm) for LB medium containing chloramphenicol, LB medium with chloramphenicol and cells containing the biobrick J3101 and the three devices (D1, D2, D3). Biological replicates originating from three different devices are presented in the rows and the technical replicates for the devices are presented in the columns.

Table 2: Absorbance values measured by the Tecan Safire2 plate reader at 600 nm (O.D. within 5% of 0.5 in a cuvette with a path length of 1 cm). The absorbance depends on the path length which is different in our plate reader attributing to the values lower than 0.5.

Table 3: Fluorescence values divided by the absorbance values for the biological and technical replicates of our three devices (D1, D2, D3) and for our negative control (LB medium with kanamycin and cells containing the promoter J3101), the arithmetic average and the standard deviation of the biological replicates (B1, B2, B3 of the respective devices).

Table 4: Fluorescence values divided by the absorbance values for the biological and technical replicates of our three devices (D1, D2, D3) and for our negative control (LB medium with kanamycin and cells containing the promoter J3101), the arithmetic average and the standard deviation of the technical replicates (T1, T2, T3 of the respective devices).

Table 5: Fluorescein standard curve. F1, F2 and F3 represent three technical replicates of every sample. From these technical replicates, we calculated the average F and the standardised average F’. The standardised average F’ is equal to the difference of the respective sample and the average F with a concentration of 0 ng/ ml fluorescein.

Table 6 : Extrapolation of GFP concentration using fluorescence measurements and fluorescein standard.