Difference between revisions of "Team:NCTU Formosa/Composite Part"
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<tr><td class="part"><span><a href="http://parts.igem.org/Part:BBa_K1694037">BBa_K1694037</a></span><br> | <tr><td class="part"><span><a href="http://parts.igem.org/Part:BBa_K1694037">BBa_K1694037</a></span><br> | ||
P<sub>cons</sub>+RBS+FadL-GBP+RBS+GFP+Ter<br> | P<sub>cons</sub>+RBS+FadL-GBP+RBS+GFP+Ter<br> | ||
| − | </td><td></td><td>With this part, we can test the GBP function by observing the green fluorescence on the gold chip. Terminator is (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>) | + | </td><td></td><td>With this part, we can test the GBP function by observing the green fluorescence on the gold chip. Terminator is (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>). |
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Latest revision as of 03:58, 19 September 2015
Composite Parts
Composite Part
| BBa_K1694013 OmpA-anti-VEGF BBa_K1694014 OmpA-anti-EGFR BBa_K1694015 OmpA-anti-HER2 | In order to change the scFv parts easily, we added a NcoI restriction site between OmpA and scFv so that we can change various scFv DNA sequence using the NcoI restriction enzyme. | |
| BBa_K1694023 Pcons+RBS+OmpA-anti-VEGF BBa_K1694024 Pcons+RBS+OmpA-anti-EGFR BBa_K1694025 Pcons+RBS+OmpA-anti-HER2 | By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv on the E.coli outer membrane continuously.
Having this part, we can co-transform with other parts in order to produce color as the detection signal. In addition, by co-transforming these different types of E.coli with different fluorescence or color as signals, we are able to create a platform which can detect multimarker and consequently achieve combination therapy. | |
| BBa_K1694033 Pcons+RBS+OmpA-anti-VEGF+RBS+GFP+Ter BBa_K1694034 Pcons+RBS+OmpA-anti-EGFR+RBS+GFP+Ter BBa_K1694035 Pcons+RBS+OmpA-anti-HER2+RBS+GFP+Ter BBa_K1694044 Pcons+RBS+OmpA-anti-EGFR+RBS+RFP+Ter BBa_K1694045 Pcons+RBS+OmpA-anti-HER2+RBS+BFP+Ter | The most commonly constructing way of composite parts is to ligate the required parts together. We also provide this kind of parts. At the back of the Lpp-OmpA-scFv part, we ligated the weaker ribosome biding site (BBa_B0030), different fluorescent protein and terminator (BBa_J61048) to make it continuously and simultaneously express the fluorescence and the scFv. We used a weak ribosome binding site to ensure that scFv's production will not be affected. | |
| BBa_K1694053 Pcons+RBS+OmpA-anti-VEGF+RBS+amilCP+Ter BBa_K1694054 Pcons+RBS+OmpA-anti-EGFR+RBS+amilCP+Ter BBa_K1694055 Pcons+RBS+OmpA-anti-HER2+RBS+amilCP+Ter | Chromoprotein is another example of what can be added when making your own probe. We constructed this part as the Pcons+RBS+OmpA-scFv+RBS+fluorescent protein+Terminator part. | |
| BBa_K1694027 Pcons+RBS+FadL-GBP | By ligating the induced promoter (BBa_J23110), ribosome binding site (BBa_B0034) and FadL-GBP, we can continuously display the GBP on the E.coli outer membrane. Then we co-transform the OmpA-scFv parts and this FadL-GBP parts into the same E.coli, hence allowing our E.coli to bind on gold chips and detect antigens simultaneously. | |
| BBa_K1694037 Pcons+RBS+FadL-GBP+RBS+GFP+Ter | With this part, we can test the GBP function by observing the green fluorescence on the gold chip. Terminator is (BBa_B0015). |
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NCTU_Formosa APOllO
National Chaio Tung University
Engineering Building 6 EF455, 1001 University Road, Hsinchu 300, Taiwan, ROC.