Difference between revisions of "Team:Toronto/Composite Part"

Line 8: Line 8:
  
 
</html>
 
</html>
−
<html>
+
{{Toronto/head}}
−
<!-- bower:css -->
+
−
<link rel="stylesheet" href="bower_components/bootstrap/dist/css/bootstrap.css" type="text/css" />
+
−
<link rel="stylesheet" href="bower_components/flexboxgrid/dist/flexboxgrid.css" type="text/css" />
+
−
<link rel="stylesheet" href="bower_components/highlightjs/styles/github.css" type="text/css" />
+
−
<!-- endbower -->
+
−
<link rel="stylesheet" href="styles/styles.css" type="text/css" />
+
−
+
−
 
+
−
<body></body>
+
−
 
+
−
 
+
−
</html>
+
−
 
+
 
<html>
 
<html>
  
 
<div id="navigation">
 
<div id="navigation">
 
<ul>
 
<ul>
−
<li><a href="index.html">home</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto">home</a></li>
−
<li><a href="team.html">team</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Team">team</a></li>
 
<li><a href="#">project</a>
 
<li><a href="#">project</a>
 
<ul>
 
<ul>
−
<li><a href="description.html">description</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Description">description</a></li>
−
<li><a href="experiments.html">experiments</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Experiments">experiments</a></li>
−
<li><a href="results.html">results</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Results">results</a></li>
−
<li><a href="design.html">design</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Design">design</a></li>
 
</ul>
 
</ul>
 
</li><li class="active"><a href="#">parts</a>
 
</li><li class="active"><a href="#">parts</a>
 
<ul>
 
<ul>
−
<li><a href="parts.html">parts</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Parts">parts</a></li>
−
<li><a href="basic_part.html">basic parts</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Basic_Part">basic parts</a></li>
−
<li class="active"><a href="composite_part.html">composite parts</a></li>
+
<li class="active"><a href="https://2015.igem.org/Team:Toronto/Composite_Part">composite parts</a></li>
−
<li><a href="part_collection.html">part collection</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Part_Collection">part collection</a></li>
 
</ul>
 
</ul>
−
</li><li><a href="notebook.html">notebook</a></li>
+
</li><li><a href="https://2015.igem.org/Team:Toronto/Notebook">notebook</a></li>
−
<li><a href="attributions.html">attributions</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Attributions">attributions</a></li>
−
<li><a href="collaborations.html">collaborations</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Collaborations">collaborations</a></li>
−
<li><a href="practices.html">human practices</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Practices">human practices</a></li>
−
<li><a href="safety.html">safety</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Safety">safety</a></li>
−
<li><a href="modeling.html">modeling</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Modeling">modeling</a></li>
−
<li><a href="measurement.html">measurement</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Measurement">measurement</a></li>
−
<li><a href="software.html">software</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Software">software</a></li>
−
<li><a href="entrepreneurship.html">entrepreneurship</a></li>
+
<li><a href="https://2015.igem.org/Team:Toronto/Entrepreneurship">entrepreneurship</a></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
 
<div class="content">
 
<div class="content">
−
<h2 id="tode-and-todf-plasmid-design">TodE and TodF Plasmid Design</h2>
 
−
<p>Pseudomonas Putida F1 species degrade toluene with the help of tod operon. Toluene dioxygenase and toluene cis-dihydrodiol dehydroxygenase transcribed from transcribed from todC1C2BA and todD genes respectively facilitate break down of toluene to 3-methylcatechol. Gerben J. Zylstra et al successfully constructed the plasmid pTDG602 containing todC1C2BAD genes. To achieve complete degradation of toluene down to carbon dioxide and water, we focused on designing a plasmid containing todE and todF genes. After cotransformation of DH10B cells with pTDG602 and our plasmid, E.Coli theoretically should contain all the genes required to achieve complete degradation.</p>
 
−
<p><img src="https://github.com/igemuoftATG/wiki2015/blob/master/images/Pathway.png" alt="alt text"></p>
 
−
<p>In order to achieve the best protein production efficiency, we codon optimized our todE and todF sequences. We used Salis RBS calculator to optimize translation efficiency. We used a tunable promoter to determine optimum transcription for DH10B cells.</p>
 
−
<p><img src="https://github.com/igemuoftATG/wiki2015/blob/master/images/biobrick%20plasmid.png" alt="alt text"></p>
 
−
<p>We also used BioBricks in the registry to design plasmids with different efficiency by using RDP kit as shown below. In this process, we used promoter BBa_J23102 and RBS BBa_B0030.</p>
 
−
<p><img src="file://localhost/Users/joannadowdell/Downloads/TodEplasmid.webp" alt="alt text"></p>
 
−
<p><img src="https://github.com/igemuoftATG/wiki2015/blob/master/images/TodFplasmid.png" alt="alt text"></p>
 
−
<p>Note: Links to iGem registry
 
−
BBa_J23102: <a href="http://parts.igem.org/Part:BBa_J23102">http://parts.igem.org/Part:BBa_J23102</a>
 
−
BBa_0030: <a href="http://parts.igem.org/Part:BBa_B0030">http://parts.igem.org/Part:BBa_B0030</a></p>
 
  
 
</div>
 
</div>
 
</html>
 
</html>
−
<html>
+
{{Toronto/footer}}
−
<script src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-AMS_HTML-full"></script>
+
−
<!-- bower:js -->
+
−
<script src="bower_components/jquery/dist/jquery.js"></script>
+
−
<script src="bower_components/bootstrap/dist/js/bootstrap.js"></script>
+
−
<script src="bower_components/highlightjs/highlight.pack.js"></script>
+
−
<!-- endbower -->
+
−
<script src="js/bundle.js"></script>
+
−
+
−
</html>
+

Revision as of 18:54, 1 October 2015