Difference between revisions of "Team:Fudan"
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<a href="javascript:void(0)"><img id="practice" class="logoimg" src="https://static.igem.org/mediawiki/2015/d/d1/Fudan_practice.png" /></a> | <a href="javascript:void(0)"><img id="practice" class="logoimg" src="https://static.igem.org/mediawiki/2015/d/d1/Fudan_practice.png" /></a> | ||
<ul id = "my_info"> | <ul id = "my_info"> | ||
| − | <li><a href=" | + | <li><a href="http://songhao.xyz/iGEM_Fudan/collaboration.html">COLLABORATIONS</a></li> |
<li><a href="http://songhao.xyz/iGEM_Fudan/notebook.html">NOTEBOOK</a></li> | <li><a href="http://songhao.xyz/iGEM_Fudan/notebook.html">NOTEBOOK</a></li> | ||
<li><a href="mailto:bertalanffy@163.com">CONTACT US: bertalanffy@163.com</a></li> | <li><a href="mailto:bertalanffy@163.com">CONTACT US: bertalanffy@163.com</a></li> | ||
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<div class = "team_title" style="display: none;"> | <div class = "team_title" style="display: none;"> | ||
| − | <p>2015 Fudan is a fabulous team. We functioned specifically and managed every aspect of the iGEM competition within our team: project design, lab work, trouble-shooting, modeling, fundraising, human practice and wiki construction. With the generous help of our advisors and instructors, we brought here our final results. Also we offered our sincere thanks and acknowledgements for all the supporters involved in helping making a fabulous team. We do appreciate it. <a href=" | + | <p>2015 Fudan is a fabulous team. We functioned specifically and managed every aspect of the iGEM competition within our team: project design, lab work, trouble-shooting, modeling, fundraising, human practice and wiki construction. With the generous help of our advisors and instructors, we brought here our final results. Also we offered our sincere thanks and acknowledgements for all the supporters involved in helping making a fabulous team. We do appreciate it. <a href="http://songhao.xyz/iGEM_Fudan/attribution.html">See more...</a></p> |
</div> | </div> | ||
<div class = "modeling_title" style="display: none;"> | <div class = "modeling_title" style="display: none;"> | ||
Revision as of 18:28, 2 October 2015
Circular RNAs (circRNAs), formed by non-sequential back-splicing of pre-mRNA transcripts, are a wide- spread form of non-coding RNA in animal cells. Various function of natural existed circRNA revealed recently shows that circRNA can be used as powerful tools in future research and health care. However, there is no toolbox to generate circRNA up to date, which restrict the research and application of circRNA. Our project focus on the devices to cyclize specific part of RNA, aiming to start a circRNA revolution.
We designed three types of devices to cyclize the RNA based on the back-splicing mechanism:the "Ouroboros"(cyclizing device based on the inverted repeat sequence in the exon-flanking region), the "Cyclizer"(proteins that accelerate RNA cyclization)and the acRNA(ssRNA that accelerate RNA cyclization).
We experimentally validate that our device can accelerate the formation of cirRNA. We also measured the half-life time of the circRNA, which proved its stability. To solidity our experiment procedure, we designed a device to report the level of mir-21 concentration and modeled the steady state of proteins to confirm our design.
Apart from our lab work, we also took active part in the educational outreach activities which has had huge influences on and off campus. As sociable participants in iGEM, we have collaboration with NYU_Shanghai, ZJU-China and NCTU Formosa, and mentored a high school team WLSA_Fudan-Shanghai. See more...
