"If you have a procedure with 10 parameters, you probably missed some." - Alan Perlis

Standard Operating Procedures (SOPs)

Figure 1: Petridishes piling up in the lab.

Throughout the project we did a lot of different experiments in the lab. To do this we needed to know what and how to do the these. For this purpose we used standard operating procedures (SOPs). All the SOPs are written by either us or some of the previous SDU iGEM Teams in collaboration with minimum one person with experience in the field of the given procedure. sometimes optimation of these SOPs is necessary, but never without asking a supervisor first. It was hereby secured that all persons was following the same procedures in the lab.

Below is listed all the SOPs used by the 2015 SDU iGEM Team:

• SDU iGEM 2015 SOP0001 - Primer design for gene Knock Out (KO) in pathogenic E.coli
• SDU iGEM 2015 SOP0002 - ​ON culture of E.coli
• SDU iGEM 2015 SOP0003 - Bacterial freezing stock
• SDU iGEM 2015 SOP0004 - ​LA plates with antibiotic
• SDU iGEM 2015 SOP0005 -​ Colony PCR with MyTaq
• SDU iGEM 2015 SOP0006 - ​TSB transformation
• SDU iGEM 2015 SOP0007 - ​Phusion PCR
• SDU iGEM 2015 SOP0008 - Gel Purification
• SDU iGEM 2015 SOP0009 - Ligation
• SDU iGEM 2015 SOP0010 - ​Fast digest
• SDU iGEM 2015 SOP0011 - ​Plasmid Miniprep
• SDU iGEM 2015 SOP0012 - ​M9 minimal medium
• SDU iGEM 2015 SOP0013 - ​Electroporation
• SDU iGEM 2015 SOP0014 - Deletion
• SDU iGEM 2015 SOP0015 - ​P1 Phage Tansduction
• SDU iGEM 2015 SOP0016 - ​Purification of RNA
• SDU iGEM 2015 SOP0017 - RNA purification
• SDU iGEM 2015 SOP0018 - Northen blotting