"The next best thing to knowing something is knowing where to find it." - Samuel Johnson

Attributions

Despite our tireless efforts we could not have done this on our own. Therefore we would like to express our gratitude and thank all.

Sponsors

• University of Southern Denmark for giving us the funds for the project, making it possible for us to participate in iGEM. In particular, we would like to thank the Faculty of Science.

• Department of Microbiology under The Deparment of Biochemistry and Molecular Biology for providing us laboratory space, equipment and always being patient and accessible.

• Integrated DNA Technologies for providing us with 20kb worth of gBlock gene fragments

Laboratory support

• Our instructors Postdoc and Ph. D. Mikkel Girke Jørgensen, Academic Assistant Tina Kronborg, Ph. D. student Thøger Jensen Krogh and Stud.cand.scient Patrick Rosendahl Andreassen
• Medical Laboratory Technician Simon Rose who provided a security course
• Postdoc Anders Boysen helped with troubleshooting in the lab

General support

• Business Scout, Ph. D. Ann Zahle - for helping us with getting to know each other through some fun team building exercizes. Furthermore she has helped us developing the business canvas model and discussing the entrepreneurship of our project.
• DTU iGEM team – for arranging the anually bio brick workshop
• Vilnius iGEM team for hosting our visit for three days
• Uppsala iGEM team for arranging and hosting the Nordic Meetup
• Associate Professor, MD Søren Thue Lillevang, Department of Clinical Immunology, Odense University Hospital, for attributing to the discussion about medical aspects
• Associate Professor Jan Nehlin, Department of Clinical Immunology, Odense University Hospital, for discussing patents and medical aspects, and suggestions to future work
• Nancy Rasmussen for helping us proof reading our wiki
• Members of the previous SDU-Denmark iGEM teams for giving feedback on our presentation and cooking for us
• Robert William Naur for proof reading on The Little Curios Story about the Curios Little Being
• Anna Valgreen for providing the illustrations, The Curios Little Story about a Curious Little Being
• Torben Hansson, Associated Professor, Institude of Chemical Engineering, Biotechnology and Enviromental Technology - for helping us with the Process Flow Diagram.
• Knud Villy Christensen, Associate Professor, MSc. Chem. Eng. PhD in Chem. Eng. - for helping advice on the Process Flow Diagram.

Technical support

• Ph. D. Thøger Jensen Krogh helped us with any problem regarding our wiki site.
• The SDU iGEM team 2013 for helping us with our wiki.

Litterature support

Antibody issue

• Liu JK1 (2014) The history of monoclonal antibody development - Progress, remaining challenges and future innovations. Sep 11;3(4):113-6. DOI: 10.1016/j.amsu.2014.09.001. (LINK)
• Dawn M Ecker, Susan Dana Jones & Howard L Levine (2015) The therapeutic monoclonal antibody market, mAbs, 7:1, 9-14, DOI: 10.4161/19420862.2015.989042. (LINK)
• Lubert Stryer JMB, John L. Tymoczko. The Immune System. Biochemistry. 7 ed: W. H. Freeman and Company; 2012.
• Cowan MK. Host Defenses II - Specific Immunity and Immunization. Microbiology - A Systems Approach. 3 ed: McGraw- Hill; 2012.
• Chippaux JP, Goyffon M. Venoms, antivenoms and immunotherapy. Toxicon. 1998;36(6):823-46.
• Clem AS. Fundamentals of vaccine immunology. J Glob Infect Dis. 2011;3(1):73-8.
• Siddiqui MZ. Monoclonal antibodies as diagnostics; an appraisal. Indian J Pharm Sci. 2010;72(1):12-7.
• Berger M, Shankar V, Vafai A. Therapeutic applications of monoclonal antibodies. Am J Med Sci. 2002;324(1):14-30.
• Payne WJ, Jr., Marshall DL, Shockley RK, Martin WJ. Clinical laboratory applications of monoclonal antibodies. Clin Microbiol Rev. 1988;1(3):313-29.
• Hnasko RM, Stanker LH. Hybridoma Technology. Methods Mol Biol. 2015;1318:15-28.
• Stills HF, Jr. Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants. ILAR journal / National Research Council, Institute of Laboratory Animal Resources. 2005;46(3):280-93.
• Rene Descartes, Meditationer over den første filosofi. Publisher: Det Lille Forlag 1. edition, published 19.04.2002.
• Jeremy Bentham, An Introduction to the Principles of Morals and Legislation (General Historical Collections). Publisher: British Library, Historical Print Editions. Published 25.03.2011
• Good Animal Practise in Science. Visited: 04.09.15. (LINK)
• Cruelty Free International. Visited 04.09.15. (LINK)
• Colas P. The eleven-year switch of peptide aptamers. Journal of biology. 2008;7(1):2.
• Borghouts C, Kunz C, Delis N, Groner B. Monomeric recombinant peptide aptamers are required for efficient intracellular uptake and target inhibition. Molecular cancer research : MCR. 2008;6(2):267-81.
• Evans D, Johnson S, Laurenson S, Davies AG, Ko Ferrigno P, Wälti C. Electrical protein detection in cell lysates using high-density peptide-aptamer microarrays. Journal of biology. 2008;7(1):3.
• Li J, Tan S, Chen X, Zhang CY, Zhang Y. Peptide aptamers with biological and therapeutic applications. Current medicinal chemistry. 2011;18(27):4215-22.
• Swiss Institute of Bioinformatics. 2015. (LINK)
• Key Benefits: Affimers have some simple but essential advantages over antibodies: Avacta Life Sciences; [updated 19- 08-2015]. (LINK)

PAST

• Karimova G, Pidoux J, Ullmann A, Ladant D. (1998) A bacterial two-hybrid system based on a reconstituted signal transduction pathway. 1998;95(10):5752-6. (LINK)
• Battesti A, Bouveret E. (2012) The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli. 2012;58(4):325-34. DOI:10.1016/j.ymeth.2012.07.018. (LINK)
• SIMON L. DOVE, J. KEITH JOUNG* & ANN HOCHSCHILD. (1997) Activation of prokaryotic transcription through arbitrary protein–protein contacts. Nature 386, 627-630 (10 April 1997). (LINK)
• J. Keith Joung, Elizabeth I. Ramm, and Carl O. Pabo (2000) A bacterial two-hybrid selection system for studying protein–DNA and protein–protein interactions. PNAS. US. vol. 97, (20 june 2000). (LINK)
• Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. (LINK)
• Borghouts C, Kunz C, Delis N, Groner B. Monomeric Recombinant Peptide Aptamers Are Required for Efficient Intracellular Uptake and Target Inhibition. Molecular Cancer Research. 2008;6(2):267-81. (LINK)
• Sigmar-Aldrich: 3xFLAG system. Visited: 16.09.15. (LINK)
• Seider, Seader, Lewin and Widagoo. Product and Process Design Principles - Synthesis, Analysis, and Evaluation; Third Edition - International Student Version; John Wiley & Sons, Inc.; 2010
• Available at: https://www.sigmaaldrich.com/denmark.html [accessed: 10.09.2015]
• Available at: https://www.neb.com/products/e8036-chitin-magnetic-beads [accessed: 13.09.2015]
• Available at: http://www.rand.org/content/dam/rand/pubs/monograph_reports/MR1325/MR1325.ch9.pdf [accessed: 15.09.2015]
• Sigma-Aldrich. Available at: http://www.sigmaaldrich.com/catalog/product/sigma/a2220?lang=en&region=DK [Accessed 1st august 2015]
• L. Yee and H. W. Blanch. Recombinant Trypsin Production in High Cell Density Fed-Batch Cultures in Escherichia coli. Biotechnology and Bioengineering. 1993; 41: 781-790
• Michael L. Shuler and Fikret Kargi. Bioprocess Engineering - Basic Concepts; second edition; Pearson Education International; 2002
• IMPACT™ Kit. 2015. New England Biolabs; [accessed 2015 Sep 11] (LINK)
• Pharmacia. Introduction to Expanded Bed Adsorption. (LINK)
• Sigma-Aldrich. (LINK)
• jeffrey Fu, David B. Wilson and Michael L. Shuler. Continuous, High Level Production and Excretion of a Plasmid- Encoded Protein by Escherichia coli in a Two-Stage Chemostat. Biotechnology and Bioengineering, 1993; 41: 937-946

Process

• World health organization, third edition, 2004 - Visited: 16.09.15. (LINK)
• Arbejdstilsynet - Visited: 16.09.15. (LINK)
• Lov om miljø og genteknologi - Visited: 16.09.15. (LINK)
• E.coli Genome Project, University of Wisconsin - Visited: 16.09.15. (LINK)
• WO 2001073108 A2 - Patent - Visited: 16.09.15. (LINK)
• The EU-parliaments directive 2009/41/EF - Visited: 16.09.15. (LINK)

Results

• One Shot® Top10 Chemically Competent E. coli. Catalog number: C4040-10. Visited: 17.09.15. (LINK)
• Kate B. Bechtol, David K. Hanzel, and Bee Choo Liang, Amersham Biosciences, Inc. - Understanding Fluorescence. (LINK)
• iGEM Parts Registry - BBa_J23119:Design. Visited: 17.09.15. (LINK)
• JCAnderson. iGEM Parts Registry - Visited: 17.09.15. (LINK)
• Brendan P. Cormack, Raphael H. Valdiviaa, Stanley Falkow - FACS-optimized mutants of the green fluorescent protein (GFP). DOI:10.1016/0378-1119(95)00685-0. (LINK)

Practices

• Jürgen Habermas, Diskurs Etik. Publisher: Det Lille Forlag, 1. edition, published 02.09.1998.
  ISBN-13: 9788790030445

What's Next

• Kim CS, et al. Cellular imaging of endosome entrapped small gold nanoparticles, 2015 Jun 10;2:306-15. doi: 10.1016/j.mex.2015.06.001. (LINK)
• Thorek DL et al. Reverse-Contrast Imaging and Targeted Radiation Therapy of Advanced Pancreatic Cancer Models. Int J Radiat Oncol Biol Phys. 2015 Jun 9. pii: S0360-3016(15)00612-4. DOI:10.1016/j.ijrobp.2015.06.001. (LINK)
• Wacker M et al. N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science. 2002 Nov 29;298(5599):1790-3. (LINK)
• Silberstein S, Lenz R, Xu C. Therapeutic Monoclonal Antibodies: What Headache Specialists Need to Know. Headache. 2015 Aug 28. DOI: 10.1111/head.12642. (LINK)
• Technical Guide for ELISA. Available from KPL, Kirkegaard & Perry Laboratories. (LINK)
• An Introduction to BiaCore's SPR Technology. Available from BiaCore.com. (LINK)
• Open WetWare contributors, "Beta-Galactosidase Assay (A better Miller)," Open WetWare27 August 2012, 23:20 UTC. (LINK)