Difference between revisions of "Team:Oxford/Experiments"

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<details>
 
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<summary><b>One-paragraph summary of our technical plan</b></summary>
 
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The project aims to investigate how bacterial biofilm-disrupting proteins can be exported from <i>E. coli</i>. The proteins Dispersin B, Microcin S, DNase, and Artilysin will be expressed from commercial pBAD expression vectors with N-terminal fusion tags to target them for export via the DsbA (Sec), YebF (Porin), and flagellar export pathways in <i>E. coli</i>. Additionally, the holin gene will be expressed under the control of bacterial quorum sensing-responsive promoters (in conjunction with the artilysin) to cause host cell lysis and release of these proteins from the cytoplasm on detection of a target cell density. Protein secretion will be monitors by the presence of a His tag on the secreted proteins detected by Western blotting of the growth medium.
 
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Revision as of 13:15, 26 October 2015