Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/6 July 2015"

Line 296: Line 296:
 
|-
 
|-
 
| 0.52g
 
| 0.52g
−
| 0.41mL
+
| 0.32g
−
| 0.78g
+
| 0.42g
 
|-
 
|-
−
| 0.32g
+
| 0.0082g
−
| 0.41 mL
+
| 0.0082g
−
| 0.48g
+
| 0.0084g
 +
|-
 +
| 0.41mL
 +
| 0.41mL
 +
| 0.42mL
 
|}
 
|}

Revision as of 23:19, 9 July 2015

Cell Lysis, continued Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)

Mass of Cell Pellet Lysozyme PMSF Solution Deoxycholic Acid
0.52g 0.32g 0.42g
0.0082g 0.0082g 0.0084g
0.41mL 0.41mL 0.42mL