Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/6 July 2015"

Line 287: Line 287:
 
</html>
 
</html>
 
<u>Cell Lysis, continued</u>
 
<u>Cell Lysis, continued</u>
 +
 
Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)
 
Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)
 
{| class="wikitable"
 
{| class="wikitable"
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| 0.41mL
 
| 0.41mL
 
| 0.48g
 
| 0.48g
 +
|-
 +
| 0.42g
 +
| 0.0084g
 +
| 0.42mL
 +
| 0.63g
 +
|-
 +
| 0.52g
 +
| 0.0084g
 +
| 0.42mL
 +
| 0.78g
 
|-
 
|-
 
| 0.42g  
 
| 0.42g  

Revision as of 00:06, 10 July 2015

Cell Lysis, continued

Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)

Mass of Cell Pellet Lysozyme PMSF Solution Deoxycholic Acid
0.52g 0.0082g 0.41mL 0.78g
0.32g 0.0082g 0.41mL 0.48g
0.42g 0.0084g 0.42mL 0.63g
0.52g 0.0084g 0.42mL 0.78g
0.42g 0.0084g 0.42mL 0.63g
0.52g 0.0084g 0.42mL 0.78g