Difference between revisions of "Team:Peking/Parts"

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                   <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
 
                   <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
 
                 </li>
 
                 </li>
−
                  
+
                 <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
 +
                </li>
 
                 <li>
 
                 <li>
 
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 
                 </li>
 
                 </li>
 
                
 
                
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
−
 
+
<!--
 
             <div id="sidebar2"class="widget widget-categories">
 
             <div id="sidebar2"class="widget widget-categories">
 
               <h4>Parts <span class="head-line"></span></h4>
 
               <h4>Parts <span class="head-line"></span></h4>
 
                 <ul>
 
                 <ul>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Contribution</a>
 +
                </li>
 +
 
                 <li>
 
                 <li>
 
                   <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
 
                   <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
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               </ul>
 
               </ul>
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             </div>
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             </div>-->
 
           </div>
 
           </div>
 
           <!--End sidebar-->
 
           <!--End sidebar-->
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<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
 
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
 
<div class="col-md-12">
 
<div class="col-md-12">
−
   <!--<table class="table table-hover table-striped" style="color:#666;font-size:14px">  
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   <table class="table table-hover table-striped" style="color:#666;font-size:14px">  
 
<thead>
 
<thead>
 
<tr>  
 
<tr>  
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<td>Composite</td>  
 
<td>Composite</td>  
 
<td>sgRNA generator</td>  
 
<td>sgRNA generator</td>  
−
<td>ZHAO Xuejin</td>
+
<td>Liu Wenchao, Xu Luze</td>  
−
</tr>
+
−
 
+
−
<tr>
+
−
  <td>&nbsp;&radic;</td>
+
−
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
+
−
<td>Composite</td>
+
−
<td>Coding sequence of dCas9-Nluc</td>
+
−
<td>ZHANG Yihao</td>
+
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>Coding sequence Cluc-dCas9</td>  
+
<td>C-luc-dCas9</td>  
−
<td>ZHANG Yihao</td>  
+
<td>Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping</td>
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td>Compostie</td>  
 
<td>Compostie</td>  
−
<td>Coding sequence of N-luc-dCas9</td>  
+
<td>N-luc-dCas9</td>  
−
<td>ZHANG Yihao</td>
+
<td>Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping</td>
 
</tr>  
 
</tr>  
  
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<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of STV-N-luc</td>  
 
<td>Coding sequence of STV-N-luc</td>  
−
<td>ZHANG Yihao</td>  
+
<td>Li Xiang, Li Jiaofeng</td>  
 
</tr>  
 
</tr>  
  
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<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of Cluc-STV</td>  
 
<td>Coding sequence of Cluc-STV</td>  
−
<td>ZHANG Yihao</td>  
+
<td>Li Xiang, Li Jiaofeng</td>  
 
</tr>  
 
</tr>  
  
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<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of Nluc416-FRB</td>
 
<td>Coding sequence of Nluc416-FRB</td>
−
<td>Xiang Li</td>   
+
<td>Wang Yu, Wang Beining, Lin Pingping, Liu Dongming</td>   
 
</tr>  
 
</tr>  
  
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<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of Nluc398-FRB</td>  
 
<td>Coding sequence of Nluc398-FRB</td>  
−
<td>Xiang Li</td>  
+
<td>Wang Yu, Wang Beining, Lin Pingping, Liu Dongming</td>
 
</tr>  
 
</tr>  
  
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<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of FKBP-Cluc398</td>  
 
<td>Coding sequence of FKBP-Cluc398</td>  
−
<td>Xiang Li</td>
+
<td>Wang Yu, Wang Beining, Lin Pingping, Liu Dongming</td>
 
</tr>  
 
</tr>  
  
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<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of FKBP-Cluc394</td>
 
<td>Coding sequence of FKBP-Cluc394</td>
−
<td>Xiang Li</td>  
+
<td>Wang Yu, Wang Beining, Lin Pingping, Liu Dongming</td>
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>Coding sequence of dCas9-C-luc</td>
+
<td>dCas9-C-luc</td>
−
<td>ZHANG Yihao</td>  
+
<td>Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-N-luc</td>
 +
<td>Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping</td>
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>delta alpha segment of lacz fused with dCas9, His tag allows for protein purification</td>
+
<td>dCas9-delta alpha</td>
−
<td>Jiaofeng Li</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>N terminal of β-lactamase fused with dCas9</td>
+
<td>dCas9-Nlact</td>
−
<td>Jiaofeng Li</th>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>  
 
<td>Compostie</td>  
 
<td>Compostie</td>  
−
<td>NFluc of lactamase fused with dCas9, His tag allows for protein purification</td>
+
<td>Nlact-dCas9</td>
−
<td>Jiaofeng Li</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>dCas9 fused with C terminal of beta-lactamase</td>
+
<td>dCas9-Clact</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>segment F[1,2] of DHFR-dCas9 fusion protein</td>
+
<td>F[1,2]-dCas9</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>fusion protein of dCas9-F[1,2]</td>
+
<td>dCas9-F[1,2]</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>DHFR F3 fragment-dCas9 fusion protein</td>
+
<td>F3-dCas9</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>dCas9 fused with F3</td>
+
<td>dCas9-F3</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>Δα segment of β-galactosidase fused with dCas9</td>
+
<td>delta alpha-dCas9</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
  
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>dCas9 fused with delta omega segment of β-galactosidase</td>
+
<td>dCas9-delta omega</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, Wei Weijia</td>  
 
</tr>  
 
</tr>  
−
</table> -->
+
</table>
−
</div>
+
<!--</div>
  
 
                 <p><b>Parts for paired dCas9 reporter system</b></p>
 
                 <p><b>Parts for paired dCas9 reporter system</b></p>
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                 <p><b>Parts for Molecular Beacon</b></p>
 
                 <p><b>Parts for Molecular Beacon</b></p>
 
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
 
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
−
</div>
+
</div>-->
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 
<div>
 
<div>

Revision as of 16:07, 17 November 2015

Peking iGEM 2015

Parts

Each part gives us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Loading.....
Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator Liu Wenchao, Xu Luze
 √ BBa_K1689009 Composite C-luc-dCas9 Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
  BBa_K1689010 Compostie N-luc-dCas9 Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
  BBa_K1689001 Coding Coding sequence of STV-N-luc Li Xiang, Li Jiaofeng
  BBa_K1689002 Coding Coding sequence of Cluc-STV Li Xiang, Li Jiaofeng
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 Wang Yu, Wang Beining, Lin Pingping, Liu Dongming
  BBa_K1689007 Composite dCas9-C-luc Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
 √ BBa_K1689008 Composite dCas9-N-luc Liu Wenchao, Wang Yu, Wang Beining, Lin Pingping
  BBa_K1689011 Composite dCas9-delta alpha ZHANG Yihao, Wei Weijia
  BBa_K1689012 Composite dCas9-Nlact ZHANG Yihao, Wei Weijia
  BBa_K1689013 Compostie Nlact-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689014 Coding dCas9-Clact ZHANG Yihao, Wei Weijia
  BBa_K1689015 Coding F[1,2]-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689016 Coding dCas9-F[1,2] ZHANG Yihao, Wei Weijia
  BBa_K1689017 Coding F3-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689018 Coding dCas9-F3 ZHANG Yihao, Wei Weijia
  BBa_K1689019 Coding delta alpha-dCas9 ZHANG Yihao, Wei Weijia
  BBa_K1689020 Coding dCas9-delta omega ZHANG Yihao, Wei Weijia

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.