Difference between revisions of "Team:Paris Saclay/Notebook/July/1"
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*Students : Johan, Seong Koo, Pauline. | *Students : Johan, Seong Koo, Pauline. | ||
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Revision as of 08:04, 10 July 2015
Contents
Lab Work
Preparation
by Johan
Plasmid Rehydratation :
by Johan Measure fluorescence : I13504 J23117 J23106 J23101
For our project : R0051 J13602 J13522 C0040
Preparation of liquid culture of DH5a with K115017
K115017 5mL LB + 5uL Cm (20mg/mL)
Competente cells preparation
By Seong Koo, Pauline, Johan
Preparation competente cells DH5a with CaCl2 cf:protocol
15mL competente cells Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C
Preparation LB Plate
by SeongKoo and Pauline
22 Plates LB + Cm (20uL/mL) 12 Plates LB + Amp (100uL/mL)
Members present:
- Instructors : Alice.
- Students : Johan, Seong Koo, Pauline.