Difference between revisions of "Team:Purdue/Description"

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<h2> Project Description </h2>
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<h1> Project Description </h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h4>Advice on writing your Project Description</h4>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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The production of some biofuels is inhibited by lignin, a complex organic polymer which physically blocks carbohydrate substrates and inhibits biomass degrading enzymes (BDEs). A common biofuel practice is to sequester lignin using energy-intensive thermal pretreatment. The recent development of a synthetic yeast that spatially separates lignin degradation products from vulnerable BDEs enables an efficient lignin breakdown system to reduce the energy input for biofuel production. By expressing enzymes from lignin-degrading species (termites and white rot fungi), we aim to establish a novel enzymatic pretreatment system in a yeast chassis. Six enzymes were selected for their ability to operate at room temperature and standard pH. The expressed enzymes were evaluated individually and in combination for lignin-degradation efficiency. Furthermore, we designed an additional vector to contain our genetically modified yeast using an oxygen-repressed killswitch.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h4>References</h4>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
 
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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Latest revision as of 00:49, 20 November 2015

Team Banner

Project Description

The production of some biofuels is inhibited by lignin, a complex organic polymer which physically blocks carbohydrate substrates and inhibits biomass degrading enzymes (BDEs). A common biofuel practice is to sequester lignin using energy-intensive thermal pretreatment. The recent development of a synthetic yeast that spatially separates lignin degradation products from vulnerable BDEs enables an efficient lignin breakdown system to reduce the energy input for biofuel production. By expressing enzymes from lignin-degrading species (termites and white rot fungi), we aim to establish a novel enzymatic pretreatment system in a yeast chassis. Six enzymes were selected for their ability to operate at room temperature and standard pH. The expressed enzymes were evaluated individually and in combination for lignin-degradation efficiency. Furthermore, we designed an additional vector to contain our genetically modified yeast using an oxygen-repressed killswitch.