Difference between revisions of "Team:Vanderbilt/Project/Achievements"
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| − | <li> | + | <li>Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits</li> |
| − | <li> | + | <li>Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements </li> |
| − | <li> | + | <li>Developed assays for measuring decreased recombination with homology-minimization software</li> |
| + | <li>Constructed optimized circuit to demonstrate improved stability with VERT technique</li> | ||
| + | <li>Validated bidirectional promoter for use with system to select against promoter mutation | ||
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| − | <li> | + | <li>Cloned five exogenous DNA repair enzymes </li> |
| − | <li> | + | <li>Incorporated KIKO vector for genomic integration and simultaneous knock-out</li> |
| − | + | <li>Designed “Incorruptible Cell” that commits suicide instead of passing on mutations | |
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Revision as of 06:57, 20 November 2015
Demons in the Code
Sequence
- Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
- Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
- Experimentally validated decreased mutation in optimized sequences with
- Multiple mutagen types (UV radiation, oxidation)
- Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)
- Established high expression of optimized sequences
- Modeling and computational strategies for further improvements and expansion of software
- Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection
Circuit
- Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
- Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
- Developed assays for measuring decreased recombination with homology-minimization software
- Constructed optimized circuit to demonstrate improved stability with VERT technique
- Validated bidirectional promoter for use with system to select against promoter mutation
Organism
- Cloned five exogenous DNA repair enzymes
- Incorporated KIKO vector for genomic integration and simultaneous knock-out
- Designed “Incorruptible Cell” that commits suicide instead of passing on mutations
