Difference between revisions of "Team:SF Bay Area DIYBio/Design"

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By talking about your design work on this page, there is one medal criterion that you can attempt to meet, and one award that you can apply for. If your team is going for a gold medal by building a functional prototype, you should tell us what you did on this page. If you are going for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Applied Design award</a>, you should also complete this page and tell us what you did.  
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1. Express Shinorine biosynthesis pathway in E. coli, also explored other UV absorbers, like GFP (!) and PABA pathway
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Measure MAA production based on UV absorption spectrum <br> <br>
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2. Improve UV resistance in E. coli using Directed Evolution!<br> <br>
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3. We need to establish how much UV to expose the cells to, to provide the right selective pressure for Directed Evolution: kill most cells, not all.<br> <br>
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4. Expose to different UV durations, and count colony forming units<br> <br>
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5. Quantify UV resistance based on kill curve of E. coli under UV exposure<br> <br>
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6. Sequence plasmid and figure out what improvements evolution came up with<br> <br>
  
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Applied Design award</a> and/or the <a href="https://2015.igem.org/Judging/Awards#Medals">functional prototype gold medal criterion</a>, you must fill out this page.</p>
 
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<p>This is a prize for the team that has developed a synthetic biology product to solve a real world problem in the most elegant way. The students will have considered how well the product addresses the problem versus other potential solutions, how the product integrates or disrupts other products and processes, and how its lifecycle can more broadly impact our lives and environments in positive and negative ways.</p>
 
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If you are working on art and design as your main project, please join the art and design track. If you are integrating art and design into the core of your main project, please apply for the award by completing this page.
 
 
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Revision as of 03:42, 21 November 2015

Design

1. Express Shinorine biosynthesis pathway in E. coli, also explored other UV absorbers, like GFP (!) and PABA pathway Measure MAA production based on UV absorption spectrum

2. Improve UV resistance in E. coli using Directed Evolution!

3. We need to establish how much UV to expose the cells to, to provide the right selective pressure for Directed Evolution: kill most cells, not all.

4. Expose to different UV durations, and count colony forming units

5. Quantify UV resistance based on kill curve of E. coli under UV exposure

6. Sequence plasmid and figure out what improvements evolution came up with