Difference between revisions of "Team:Concordia/ResultsCloning"

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<p style="font-size:18px; font-family: Tahoma">
 
<p style="font-size:18px; font-family: Tahoma">
  
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We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones.</p>
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We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, into a single construct and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones. Gel lanes 21 to 26 showcase 3 enzymes, SacC, ADH2, ALD2, that were cloned into backbones. In total, 13 original parts were added by our team to the iGEM Parts registry.</p>
  
 
<img src="https://static.igem.org/mediawiki/2015/0/07/Concordia-gel.jpeg" class= "img-thumbnail" alt="Gel electrophoresis" align="left" style="margin-right: 20px; height:500px">
 
<img src="https://static.igem.org/mediawiki/2015/0/07/Concordia-gel.jpeg" class= "img-thumbnail" alt="Gel electrophoresis" align="left" style="margin-right: 20px; height:500px">
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<table class="table table-bordered" style="background-color:white">
 +
    <thead style="background-color:#d3d3d3">
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      <tr>
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        <th>Gel Lanes</th>
 +
        <th>Part</th>
 +
        <th>Registry #</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
      <tr>
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        <td>1,2</td>
 +
        <td>PnisA</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830001">BBa_K1830001</a></td>
 +
      </tr>
 +
      <tr>
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        <td>3,4</td>
 +
        <td>SPusp45</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830002">BBa_K1830002</a></td>
 +
      </tr>
 +
      <tr>
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        <td>5,6</td>
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        <td>CBD</td>
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        <td><a href="http://parts.igem.org/Part:BBa_K1830003">BBa_K1830003</a></td>
 +
      </tr>
 +
      <tr>
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        <td>7,8</td>
 +
        <td>Coh1c3</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830004">BBa_K1830004</a></td>
 +
      </tr>
 +
      <tr>
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        <td>9,10</td>
 +
        <td>Coh2S1</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830005">BBa_K1830005</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>11,12</td>
 +
        <td>CWAm6</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830006">BBa_K1830006</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>13,14</td>
 +
        <td>T1T2 terminator</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830007">BBa_K1830007</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>15,16</td>
 +
        <td>Lactis shuttle</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830008">BBa_K1830008</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>17,18</td>
 +
        <td>Dock1</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830009">BBa_K1830009</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>19,20</td>
 +
        <td>XDock2</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830010">BBa_K1830010</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>21,22</td>
 +
        <td>SacC</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830015">BBa_K1830015</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>23,24</td>
 +
        <td>ADH2 (alcohol dehydrogenase)</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830013">BBa_K1830013</a></td>
 +
      </tr>
 +
      <tr>
 +
        <td>25,26</td>
 +
        <td>ALD2 (aldehyde dehydrogenase)</td>
 +
        <td><a href="http://parts.igem.org/Part:BBa_K1830014">BBa_K1830014</a></td>
 +
      </tr>
 +
</tbody>
 +
</table>
  
  

Revision as of 03:59, 21 November 2015

Results of Cloning

Gel Electrophoresis of Team Parts

Scaffold genes

We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, into a single construct and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones. Gel lanes 21 to 26 showcase 3 enzymes, SacC, ADH2, ALD2, that were cloned into backbones. In total, 13 original parts were added by our team to the iGEM Parts registry.

Gel electrophoresis
Gel Lanes Part Registry #
1,2 PnisA BBa_K1830001
3,4 SPusp45 BBa_K1830002
5,6 CBD BBa_K1830003
7,8 Coh1c3 BBa_K1830004
9,10 Coh2S1 BBa_K1830005
11,12 CWAm6 BBa_K1830006
13,14 T1T2 terminator BBa_K1830007
15,16 Lactis shuttle BBa_K1830008
17,18 Dock1 BBa_K1830009
19,20 XDock2 BBa_K1830010
21,22 SacC BBa_K1830015
23,24 ADH2 (alcohol dehydrogenase) BBa_K1830013
25,26 ALD2 (aldehyde dehydrogenase) BBa_K1830014