Difference between revisions of "Team:Concordia/ResultsCloning"
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| − | We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, into a single construct and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones. Gel lanes 21 to 26 showcase 3 enzymes, SacC, ADH2, ALD2, that were cloned into backbones. In total, 13 original parts were added by our team to the iGEM Parts registry. | + | We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, into a single construct and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones. Gel lanes 21 to 26 showcase 3 enzymes, SacC, ADH2, ALD2, that were cloned into backbones. In total, 13 original parts were added by our team to the iGEM Parts registry. |
<img src="https://static.igem.org/mediawiki/2015/0/07/Concordia-gel.jpeg" class= "img-thumbnail" alt="Gel electrophoresis" align="left" style="margin-right: 20px; height:500px"> | <img src="https://static.igem.org/mediawiki/2015/0/07/Concordia-gel.jpeg" class= "img-thumbnail" alt="Gel electrophoresis" align="left" style="margin-right: 20px; height:500px"> | ||
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| + | <table class="table table-bordered" style="background-color:white; align:right; width:auto"> | ||
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Revision as of 04:00, 21 November 2015
Results of Cloning
Gel Electrophoresis of Team Parts
We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, into a single construct and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones. Gel lanes 21 to 26 showcase 3 enzymes, SacC, ADH2, ALD2, that were cloned into backbones. In total, 13 original parts were added by our team to the iGEM Parts registry.
| Gel Lanes | Part | Registry # |
|---|---|---|
| 1,2 | PnisA | BBa_K1830001 |
| 3,4 | SPusp45 | BBa_K1830002 |
| 5,6 | CBD | BBa_K1830003 |
| 7,8 | Coh1c3 | BBa_K1830004 |
| 9,10 | Coh2S1 | BBa_K1830005 |
| 11,12 | CWAm6 | BBa_K1830006 |
| 13,14 | T1T2 terminator | BBa_K1830007 |
| 15,16 | Lactis shuttle | BBa_K1830008 |
| 17,18 | Dock1 | BBa_K1830009 |
| 19,20 | XDock2 | BBa_K1830010 |
| 21,22 | SacC | BBa_K1830015 |
| 23,24 | ADH2 (alcohol dehydrogenase) | BBa_K1830013 |
| 25,26 | ALD2 (aldehyde dehydrogenase) | BBa_K1830014 |