Difference between revisions of "Team:SF Bay Area DIYBio/Results"
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4 log decrease in viable cells after 75min for HB101, 30min for pGLO | 4 log decrease in viable cells after 75min for HB101, 30min for pGLO | ||
If anything, pGLO has a negative effect on UV resistance! | If anything, pGLO has a negative effect on UV resistance! | ||
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| + | <h2> Accomplishments </h2> | ||
| + | <p> | ||
| + | Researched UV sources and built UV exposure rig to mimic solar UV <br><br> | ||
| + | Determined exposure level needed for >4 log decrease in CFU <br><br> | ||
| + | Demonstrated GFP is NOT an effective UV protectant for E.coli <br><br> | ||
| + | Synthesized and transformed A. variabilis shinorine biosynthesis genes <br><br> | ||
| + | Submitted one gene (Ava_3856) to Parts Registry <br><br> | ||
| + | Demonstrated that we produced UV absorbing compounds <br><br> | ||
</p> | </p> | ||
</html> | </html> | ||
Revision as of 04:26, 21 November 2015
Project Results
UV kill curve was generated for E.coli HB101, to establish baseline exposure for Directed Evolution
We also tested HB101 + pGLO plasmid, to check if GFP has a protective effect.
4 log decrease in viable cells after 75min for HB101, 30min for pGLO
If anything, pGLO has a negative effect on UV resistance!
Accomplishments
Researched UV sources and built UV exposure rig to mimic solar UV
Determined exposure level needed for >4 log decrease in CFU
Demonstrated GFP is NOT an effective UV protectant for E.coli
Synthesized and transformed A. variabilis shinorine biosynthesis genes
Submitted one gene (Ava_3856) to Parts Registry
Demonstrated that we produced UV absorbing compounds