Difference between revisions of "Template:Team:Groningen/CONTENT/PROTOCOLS/Restriction"

(Created page with "{{Team:Groningen/TEMPLATES/STEP |title=Restriction |content=<html> <div class="object data" id="tbl1"> <div class="wrapper"> <div class="header"> <div class="field fw3">Com...")
 
 
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<div class="record">
 
<div class="record">
 
<div class="field fw3">Water, nuclease free*</div>
 
<div class="field fw3">Water, nuclease free*</div>
−
<div class="field fw3">up to 20 μl</div>
+
<div class="field fw3">up to 20 μL</div>
−
<div class="field fw3">up to 20 μl</div>
+
<div class="field fw3">up to 20 μL</div>
−
                 <div class="field fw3">up to 50 μl</div>
+
                 <div class="field fw3">up to 50 μL</div>
 
</div>
 
</div>
 
<div class="record">
 
<div class="record">
 
<div class="field fw3">10x NEB 2.1 buffer</div>
 
<div class="field fw3">10x NEB 2.1 buffer</div>
−
<div class="field fw3">2 μl</div>
+
<div class="field fw3">2 μL</div>
−
<div class="field fw3">2 μl**</div>
+
<div class="field fw3">2 μL**</div>
−
                 <div class="field fw3">5 μl</div>
+
                 <div class="field fw3">5 μL</div>
 
</div>
 
</div>
 
<div class="record">
 
<div class="record">
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<div class="record">
 
<div class="record">
 
<div class="field fw3">NEB Enzyme</div>
 
<div class="field fw3">NEB Enzyme</div>
−
<div class="field fw3">0.5 μl</div>
+
<div class="field fw3">0.5 μL</div>
−
<div class="field fw3">0.5 μl</div>
+
<div class="field fw3">0.5 μL</div>
−
                 <div class="field fw3">0.5 μl</div>
+
                 <div class="field fw3">0.5 μL</div>
 
</div>
 
</div>
 
         <div class="record">
 
         <div class="record">
 
<div class="field fw3">Total Volume</div>
 
<div class="field fw3">Total Volume</div>
−
<div class="field fw3">20 μl</div>
+
<div class="field fw3">20 μL</div>
−
<div class="field fw3">20 μl</div>
+
<div class="field fw3">20 μL</div>
−
                 <div class="field fw3">20 μl</div>
+
                 <div class="field fw3">20 μL</div>
 
</div>
 
</div>
 
</div>
 
</div>

Latest revision as of 21:49, 21 November 2015

Restriction

Component
Plasmid DNA
Unpurified Product
Genomic DNA
Water, nuclease free*
up to 20 μL
up to 20 μL
up to 50 μL
10x NEB 2.1 buffer
2 μL
2 μL**
5 μL
DNA
up to 1 μg
up to 0.2 μg
up to 5 μg
NEB Enzyme
0.5 μL
0.5 μL
0.5 μL
Total Volume
20 μL
20 μL
20 μL
Restriction.