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Latest revision as of 23:07, 21 November 2015
Restriction thrC locus 2(th14)
Digestion of PCR product thrC locus 2
Extra integration of DNA in thrC locus
Digested PCR product
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 3 September 2015 - 00:00, 3 September 2015
The PCR products were digested with the SacI and PstI restriction enzymes. The restriction sample was put on a 1% agarose gel. After running, the band was cut out of the gel and purified with the PCR purification kit from Thermo Scientific.