Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Transformation th17"
(Created page with "{{Team:Groningen/TEMPLATES/EXPERIMENT |title=Transformation thrC locus 2 in thrC plasmid A (th17) |protocol=Heat shock transformation |protocolurl=Heat_shock_transformation...") |
|||
| Line 3: | Line 3: | ||
|protocol=Heat shock transformation | |protocol=Heat shock transformation | ||
|protocolurl=Heat_shock_transformation | |protocolurl=Heat_shock_transformation | ||
| − | |goal= | + | |goal=Extra integration of DNA in thrC locus |
| − | |description= | + | |description=Transformation of thrC locus 2 in thrC plasmid A in <i>E. coli</i> |
| − | |conclusion=Transformed | + | |conclusion=Transformed <i>E. coli</i> cells. |
}} | }} | ||
Latest revision as of 23:11, 21 November 2015
Transformation thrC locus 2 in thrC plasmid A (th17)
Transformation of thrC locus 2 in thrC plasmid A in E. coli
Extra integration of DNA in thrC locus
Transformed E. coli cells.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
00:00, 3 September 2015 - 00:00, 3 September 2015
A transformation for both ligated products was performed in NEB E. coli competent cells. The cells were plated on LB plates with corresponding antibiotics.