Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Restriction thrC compleet, tasA, bslA and slrR Inserts (th21)"

 
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|executor=Marieke Mulder
 
|executor=Marieke Mulder
 
|content=
 
|content=
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<div class="text">
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<div class="text">The following components were used.</div>
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the following samples were digested.
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<div class="object data" id="tbl1">
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construct
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<div class="wrapper">
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restriction enzymes
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<div class="header">
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ThrC backbone with EcoRI and PstI.
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<div class="field fw3">Construct</div>
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Salt promoter with TasA in pSB1C3 with EcoRI and PstI.
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<div class="field fw7">Restriction enzymes</div>
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Salt promoter with YuaB in pSB1C3 with EcoRI and PstI.
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</div>
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SlrR in pSB1C3 with EcoRI and PstI.
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<div class="record">
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<div class="field fw3">ThrC backbone</div>
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<div class="field fw7">EcoRI and PstI</div>
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</div>
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</div>
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<div class="caption">Restriction.</div>
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</div>
 
   
 
   
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restriction were performed for 2 hours at 37°C in stopped by heating at 80°C for 20 min.
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<div class="text"> The restriction was performed for 2 hours at 37 °C in stopped by heating at 80°C for 20 min.</div>
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</div>
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Latest revision as of 23:21, 21 November 2015

00:00, 6 September 2015 - 00:00, 6 September 2015
The following components were used.
Construct
Restriction enzymes
ThrC backbone
EcoRI and PstI
Restriction.
The restriction was performed for 2 hours at 37 °C in stopped by heating at 80°C for 20 min.
Marieke Mulder