Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Ligation with thrC complete with inserts th22"

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The ligation of the thrC with inserts included ligating thrC with the Psalt + tasA and with Psalt + bslA. Finally slrR was also ligated in the thrC backbone. The ligations were incubated for 1 hour at room temperature.</div>
 
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Ligation thrC with inserts
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ThrC backbone with Salt promoter with TasA.
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ThrC backbone with Salt promoter with YuaB.
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ThrC backbone with SlrR.
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Ligations were incubated for 1 hour at RT
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Latest revision as of 23:25, 21 November 2015

00:00, 6 September 2015 - 00:00, 6 September 2015
The ligation of the thrC with inserts included ligating thrC with the Psalt + tasA and with Psalt + bslA. Finally slrR was also ligated in the thrC backbone. The ligations were incubated for 1 hour at room temperature.
Marieke Mulder