Performed a transformation for both ligated products in NEB alpha competent cells.
Cells were plated on LB plates with corresponding antibiotics
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|protocol=Heat shock transformation | |protocol=Heat shock transformation | ||
|protocolurl=Heat_shock_transformation | |protocolurl=Heat_shock_transformation | ||
− | |goal= | + | |goal=Extra integration of DNA in thrC locus |
− | |description= | + | |description=Transformed the thrC complete with tasA, bslA or slrR |
− | |conclusion= | + | |conclusion=Transformed cells |
}} | }} |
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
Performed a transformation for both ligated products in NEB alpha competent cells.
Cells were plated on LB plates with corresponding antibiotics