Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Transformation th23"

(Created page with "{{Team:Groningen/TEMPLATES/EXPERIMENT |title=Transformation of thrC complete with inserts in E. coli (th23) |protocol=Heat shock transformation |protocolurl=Heat_shock_tran...")
 
 
Line 3: Line 3:
 
|protocol=Heat shock transformation
 
|protocol=Heat shock transformation
 
|protocolurl=Heat_shock_transformation
 
|protocolurl=Heat_shock_transformation
|goal=extra integration of DNA in thrC locus
+
|goal=Extra integration of DNA in thrC locus
|description= transformed the thrC complete with tasA, bslA or slrR
+
|description=Transformed the thrC complete with tasA, bslA or slrR
|conclusion= transformed cells
+
|conclusion=Transformed cells
 
}}
 
}}

Latest revision as of 23:26, 21 November 2015

Transformation of thrC complete with inserts in E. coli (th23)
Transformed the thrC complete with tasA, bslA or slrR
Extra integration of DNA in thrC locus
Transformed cells

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>


00:00, 6 September 2015 - 00:00, 6 September 2015

Performed a transformation for both ligated products in NEB alpha competent cells.

Cells were plated on LB plates with corresponding antibiotics

Marieke Mulder