Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Restriction th3"
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{{Team:Groningen/TEMPLATES/EXPERIMENT | {{Team:Groningen/TEMPLATES/EXPERIMENT | ||
|title= Restriction (th3) | |title= Restriction (th3) | ||
|protocol=Restriction | |protocol=Restriction | ||
|protocolurl=restriction | |protocolurl=restriction | ||
| − | |goal= | + | |goal=Extra integration of DNA in thrC locus |
|description=Digestion of pdg1664 | |description=Digestion of pdg1664 | ||
| − | |conclusion=Plasmid was | + | |conclusion=Plasmid was digested and purified |
}} | }} | ||
Latest revision as of 23:34, 21 November 2015
Restriction (th3)
Digestion of pdg1664
Extra integration of DNA in thrC locus
Plasmid was digested and purified
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
pdg1664 plasmid DNA was digested with EcoRI and AatII for 2 hours at 37 °C. The restriction samples were run on a 1% agarose gel and correct bands were cut out of the gel and purified with the PCR purification kit from Thermo Scientific.