Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Transform k23 th5"

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|protocol=Heat shock transformation
 
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|goal=extra integration of DNA in thrC locus
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|goal=Extra integration of DNA in thrC locus
|description= transforming thrC locus in K823023
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|description=Transforming thrC locus in K823023
|conclusion= thrC locus in K832023 was transformed in <i> E. coli</1>
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|conclusion=thrC locus in K832023 was transformed in <i> E. coli</1>
 
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Latest revision as of 23:36, 21 November 2015

Transformation pdg1664 in k823023 (th5)
Transforming thrC locus in K823023
Extra integration of DNA in thrC locus
thrC locus in K832023 was transformed in E. coli</1></div>

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>

00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
Performed a transformation for the ligation of the thrC locus from pdg1664 and K823023 products in NEB alpha competent cells. The cells were plated on LB plates with corresponding antibiotics.
Marieke


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