Difference between revisions of "Team:IIT Kharagpur/Notebook"

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<h1>Content for the Notebook Page</h1>
 
<h1>Content for the Notebook Page</h1>
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<p><h2 class="heading">(Log of the work done- Day to day, Project Component
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Wise,<br> and/or any other characterization)</h2></p>
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<p><h2 class="heading"><u>WEEK-1( July 6 - July 10 )</u></h2></p>
 
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<h2>6th July 2015</h2>
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<ul>
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<li>LB media prepared (500 ml), autoclaved and stored at 4 degrees.</li>
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<li>250 ml of LB agar prepared and poured into the plates (chloramphenicol antibiotic
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used- concentration 1 &micro;l/ml).</li>
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</ul>
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</p>
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</p>
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<h2>7th July 2015</h2>
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<ul>
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<li>DNA from wells dissolved into 10 &micro;l distilled water.<u> DNA kit plate instructions</u></li>
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<li>Transformed 4&micro;l of DNA into 100&micro;l of E.Coli DH5&alpha;.<u> transformation procedure</u></li>
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<li>100&micro;l of transformed cells were plated on chloramphenicol resistant plates.and left
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overnight to grow.</li>
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</ul>
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<h2>8th July 2015</h2>
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<p>
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<li>Colonies for luxPR promoter (BBa_K546003) constructs were observed. A colony
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was picked and inoculated in 3 ml culture for glycerol stock preparation.</li>
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<li>No colony observed for luxR protein (BBa_R6002) construct.</li>
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</ul>
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</p>
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<h2>9th July 2015</h2>
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<p>
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<ul>
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<li>Transformation repeated for luxR protein construct. <u>transformation procedure</u></li>
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<li>Culture prepared for plasmid isolation for luxPR promoter. Colony was picked and
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inoculated in 10 ml culture.</li>
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</ul>
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</p>
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</p>
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<h2>10th July 2015</h2>
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<ul>
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<li>No colony observed for luxR protein construct.</li>
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<li>Plasmid isolation done for luxPR promoter.</li>
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<li>Gel electrophoresis done for luxPR promoter.</li>
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<li>Lane 7 : 1kb marker<br>
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            Lane 8 : luxPR promoter</li>
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</ul>
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Revision as of 18:27, 11 July 2015

Content for the Notebook Page

(Log of the work done- Day to day, Project Component Wise,
and/or any other characterization)

WEEK-1( July 6 - July 10 )

6th July 2015

  • LB media prepared (500 ml), autoclaved and stored at 4 degrees.
  • 250 ml of LB agar prepared and poured into the plates (chloramphenicol antibiotic used- concentration 1 µl/ml).

7th July 2015

  • DNA from wells dissolved into 10 µl distilled water. DNA kit plate instructions
  • Transformed 4µl of DNA into 100µl of E.Coli DH5α. transformation procedure
  • 100µl of transformed cells were plated on chloramphenicol resistant plates.and left overnight to grow.

8th July 2015

  • Colonies for luxPR promoter (BBa_K546003) constructs were observed. A colony was picked and inoculated in 3 ml culture for glycerol stock preparation.
  • No colony observed for luxR protein (BBa_R6002) construct.

9th July 2015

  • Transformation repeated for luxR protein construct. transformation procedure
  • Culture prepared for plasmid isolation for luxPR promoter. Colony was picked and inoculated in 10 ml culture.

10th July 2015

  • No colony observed for luxR protein construct.
  • Plasmid isolation done for luxPR promoter.
  • Gel electrophoresis done for luxPR promoter.
  • Lane 7 : 1kb marker
    Lane 8 : luxPR promoter